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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F12%3A43894756%21RIV13-GA0-22330___
rdf:type
skos:Concept n5:Vysledek
dcterms:description
Bread represents an important source of trace elements in the human diet. This study is focused on home prepared bread in the Czech Republic. The amounts of Cu, Mo, Mn, Ni and Zn (total and soluble in Tris-HCl buffer, pH 7.5), Cd, Co, Fe, Pb and Tl (total only) as well as Hg (total and soluble in mercaptoethanol-HCl mixture) in raw materials and baked bread were determined using ICP-MS. Moreover, the speciation of elements was investigated using HPLC/ICP-MS. Isolated peptide ligands of the trace elements were analysed for amino acids and characterised by MALDI-MS. The concentrations of all elements were in accordance with Czech legislation. The solubility of the Ni species was not affected by the baking process, whereas the solubilities of Mo, Mn and Zn species decreased. Soluble mercury was found only in the inorganic form. The soluble species of Cu, Mo, Mn, Ni and Zn were found in two fractions with the apparent molecular weights of 1-2 kDa and 4-5 kDa. Ligands of trace metals isolated from these fractions contained appreciable amounts of Asx, Glx, Gly, Ser and Cys. No phytochelatin-like peptides were found in the MALDI-MS spectra of isolated ligands,. Using MALDI-MS/MS, the partial amino-acids sequences of peptide ligands were obtained, and the linkages of peptides and saccharides confirmed. The MS analysis of the trypsin digest of the medium molecular weight fraction revealed several proteins rich in cysteine (e.g., barwin and amylase inhibitors). Bread represents an important source of trace elements in the human diet. This study is focused on home prepared bread in the Czech Republic. The amounts of Cu, Mo, Mn, Ni and Zn (total and soluble in Tris-HCl buffer, pH 7.5), Cd, Co, Fe, Pb and Tl (total only) as well as Hg (total and soluble in mercaptoethanol-HCl mixture) in raw materials and baked bread were determined using ICP-MS. Moreover, the speciation of elements was investigated using HPLC/ICP-MS. Isolated peptide ligands of the trace elements were analysed for amino acids and characterised by MALDI-MS. The concentrations of all elements were in accordance with Czech legislation. The solubility of the Ni species was not affected by the baking process, whereas the solubilities of Mo, Mn and Zn species decreased. Soluble mercury was found only in the inorganic form. The soluble species of Cu, Mo, Mn, Ni and Zn were found in two fractions with the apparent molecular weights of 1-2 kDa and 4-5 kDa. Ligands of trace metals isolated from these fractions contained appreciable amounts of Asx, Glx, Gly, Ser and Cys. No phytochelatin-like peptides were found in the MALDI-MS spectra of isolated ligands,. Using MALDI-MS/MS, the partial amino-acids sequences of peptide ligands were obtained, and the linkages of peptides and saccharides confirmed. The MS analysis of the trypsin digest of the medium molecular weight fraction revealed several proteins rich in cysteine (e.g., barwin and amylase inhibitors).
dcterms:title
Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread
skos:prefLabel
Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread Analyses of trace metals, peptide ligands of trace metals and mercury speciation in home prepared bread
skos:notation
RIV/60461373:22330/12:43894756!RIV13-GA0-22330___
n5:predkladatel
n6:orjk%3A22330
n3:aktivita
n17:Z n17:S n17:P
n3:aktivity
P(GA525/07/0903), P(LC06034), S, Z(MSM6046137307)
n3:cisloPeriodika
2
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n10:8440646 n10:1120530
n3:druhVysledku
n20:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
122060
n3:idVysledku
RIV/60461373:22330/12:43894756
n3:jazykVysledku
n21:eng
n3:klicovaSlova
proteomics; mercury; trace metals; speciation; bread
n3:klicoveSlovo
n15:bread n15:proteomics n15:trace%20metals n15:speciation n15:mercury
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[04B759C9A322]
n3:nazevZdroje
Chemical Speciation and Bioavailability
n3:obor
n4:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n19:GA525%2F07%2F0903 n19:LC06034
n3:rokUplatneniVysledku
n13:2012
n3:svazekPeriodika
24
n3:tvurceVysledku
Kačer, Petr Mestek, Oto Komínková, Jana Šantrůček, Jiří Mališová, Kateřina Koplík, Richard
n3:wos
000305370400002
n3:zamer
n18:MSM6046137307
s:issn
0954-2299
s:numberOfPages
10
n12:doi
10.3184/095422912X13325261626531
n16:organizacniJednotka
22330