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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F12%3A43893693%21RIV13-GA0-22330___
rdf:type
n16:Vysledek skos:Concept
dcterms:description
The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemical assay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level differed among transgenic lines. Based on results of degradation experiment with 2,3-dihydroxybiphenyl, the transgenic lines G1, H1 and L1 were transformed by plasmid carrying yeast methallothionein gene CUP in fusion with histidine tail. The methallothioneins are known for their ability to bind heavy metals, histidine tail is other binding domain enhancing the effect. The transformation of transgenic plants was mediated by Agrobacterium tumefaciens.Transgenic plants containing both bphC and CUP gene were observed and the transcription of genes was confirmed using transient expression as well as permanent transformation. The aim of this work is the preparation of transgenic plant resistant to both organic and inorganic pollutants. Firstly transgenic plants containing bacterial bphC gene were prepared. The bphC gene is coding for 2,3- dihydroxybiphenyl-1,2-dioxygenase cleaving the biphenyl ring to form a product which could be further metabolized. The presence of bphC gene in first and second filial generation of transgenic plants was detected on the DNA and RNA level using PCR or RT-PCR methods. Transgenic plants contain the bphC gene in fusion with genes for detection markers GUS (gene for beta-glucuronidase) and LUC (gene for luciferase) and also bphC gene with histidine tail. The expression of the bphC/GUS and the bphC/LUC genes in plants was tested by histochemical assay proving the expression of detection markers. Also the expression of the bphC/His gene was verified after purification of the enzyme by affinity chromatography followed by Western blot and immunochemical assay. The expression level differed among transgenic lines. Based on results of degradation experiment with 2,3-dihydroxybiphenyl, the transgenic lines G1, H1 and L1 were transformed by plasmid carrying yeast methallothionein gene CUP in fusion with histidine tail. The methallothioneins are known for their ability to bind heavy metals, histidine tail is other binding domain enhancing the effect. The transformation of transgenic plants was mediated by Agrobacterium tumefaciens.Transgenic plants containing both bphC and CUP gene were observed and the transcription of genes was confirmed using transient expression as well as permanent transformation.
dcterms:title
Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes
skos:prefLabel
Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes Preparation of super-transgenic plants expressing both dioxygenase and metallothionein genes
skos:notation
RIV/60461373:22330/12:43893693!RIV13-GA0-22330___
n16:predkladatel
n21:orjk%3A22330
n3:aktivita
n7:S n7:P
n3:aktivity
P(GPP501/12/P521), P(LH12087), S
n3:dodaniDat
n4:2013
n3:domaciTvurceVysledku
n6:5015804 n6:9344721 n6:4811526 n6:1304984 n6:9615849 n6:5180953
n3:druhVysledku
n14:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n22:predkladatel
n3:idSjednocenehoVysledku
161366
n3:idVysledku
RIV/60461373:22330/12:43893693
n3:jazykVysledku
n10:eng
n3:klicovaSlova
phytoremediation; metallothionein; BphC; transgenic plants
n3:klicoveSlovo
n11:BphC n11:metallothionein n11:transgenic%20plants n11:phytoremediation
n3:kontrolniKodProRIV
[18968E20C924]
n3:mistoKonaniAkce
Praha
n3:mistoVydani
Praha
n3:nazevZdroje
5th International Symposium on Biosorption and Bioremediation
n3:obor
n20:DK
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
7
n3:projekt
n12:LH12087 n12:GPP501%2F12%2FP521
n3:rokUplatneniVysledku
n4:2012
n3:tvurceVysledku
Neumannová, E. Nováková, Martina Macek, Tomáš Macková, Martina Viktorová, Jitka Fišer, Jan Bečvářová, Zuzana
n3:typAkce
n17:CST
n3:zahajeniAkce
2012-06-24+02:00
s:numberOfPages
4
n18:hasPublisher
Vysoká škola chemicko-technologická v Praze
n5:isbn
978-80-7080-825-2
n15:organizacniJednotka
22330