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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F10%3A00024459%21RIV12-AV0-22330___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Visfatin was originally described as an adipokine with insulin mimetic effects. Recently, it was found that visfatin is identical with the Nampt (nicotinamide phosphoribosyltransferase) gene that codes for an intra- and extracellular NAD biosynthetic enzyme and is predominantly expressed outside the adipose tissue. In the current study, we found strong protein and mRNA expression of visfatin in rat heart, liver, kidney, and muscle, while the expression of visfatin in visceral fat was significantly lower and undetectable in subcutaneous fat. The insulin-mimetic effects of visfatin (extracellular form of Nampt or eNampt) are controversial and even less is known about autocrine effects of visfatin (intracellular form of Nampt or iNampt). Since liver plays a major role in glucose metabolism, we studied visfatin effects on insulin-stimulated cellular glucose uptake in Fao rat hepatocytes using RNA interference (RNAi). RNAi-mediated downregulation of visfatin expression in Fao cells was associated with significantly reduced NAD biosynthesis (0.3+/-0.01 vs. 0.5+/-0.01 mmol/h/g, P{0.05) and with significantly decreased incremental glucose uptake after stimulation with insulin when compared to controls with normal expression of visfatin (0.6+/-0.2 vs. 2.2+/-0.5 nnmol/g/2 h, P=0.02). These results provide evidence that visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis. Visfatin was originally described as an adipokine with insulin mimetic effects. Recently, it was found that visfatin is identical with the Nampt (nicotinamide phosphoribosyltransferase) gene that codes for an intra- and extracellular NAD biosynthetic enzyme and is predominantly expressed outside the adipose tissue. In the current study, we found strong protein and mRNA expression of visfatin in rat heart, liver, kidney, and muscle, while the expression of visfatin in visceral fat was significantly lower and undetectable in subcutaneous fat. The insulin-mimetic effects of visfatin (extracellular form of Nampt or eNampt) are controversial and even less is known about autocrine effects of visfatin (intracellular form of Nampt or iNampt). Since liver plays a major role in glucose metabolism, we studied visfatin effects on insulin-stimulated cellular glucose uptake in Fao rat hepatocytes using RNA interference (RNAi). RNAi-mediated downregulation of visfatin expression in Fao cells was associated with significantly reduced NAD biosynthesis (0.3+/-0.01 vs. 0.5+/-0.01 mmol/h/g, P{0.05) and with significantly decreased incremental glucose uptake after stimulation with insulin when compared to controls with normal expression of visfatin (0.6+/-0.2 vs. 2.2+/-0.5 nnmol/g/2 h, P=0.02). These results provide evidence that visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis.
dcterms:title
Autocrine effects of visfatin on hepatocyte sensitivity to insulin action. Autocrine effects of visfatin on hepatocyte sensitivity to insulin action.
skos:prefLabel
Autocrine effects of visfatin on hepatocyte sensitivity to insulin action. Autocrine effects of visfatin on hepatocyte sensitivity to insulin action.
skos:notation
RIV/60461373:22330/10:00024459!RIV12-AV0-22330___
n4:aktivita
n6:P n6:Z
n4:aktivity
P(IAA500110805), P(ME08006), P(NR9359), P(NR9387), Z(AV0Z50110509), Z(AV0Z50200510)
n4:cisloPeriodika
4
n4:dodaniDat
n12:2012
n4:domaciTvurceVysledku
n7:3931390 n7:8225044 n7:2472643
n4:druhVysledku
n16:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
248139
n4:idVysledku
RIV/60461373:22330/10:00024459
n4:jazykVysledku
n8:eng
n4:klicovaSlova
Visfatin, Fao hepatocytes, Insulin resistance, RNA interference
n4:klicoveSlovo
n5:Visfatin n5:Insulin%20resistance n5:RNA%20interference n5:Fao%20hepatocytes
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[4A1587862979]
n4:nazevZdroje
Physiological Research
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
8
n4:projekt
n18:NR9359 n18:NR9387 n18:ME08006 n18:IAA500110805
n4:rokUplatneniVysledku
n12:2010
n4:svazekPeriodika
59
n4:tvurceVysledku
Pravenec, M. Škop, Vojtěch Kontrová, K. Zídek, V. Kazdová, L. Mikulík, K. Sajdok, Jiří Zídková, Jarmila
n4:wos
000282054700016
n4:zamer
n17:AV0Z50110509 n17:AV0Z50200510
s:issn
0862-8408
s:numberOfPages
4
n10:organizacniJednotka
22330