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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F10%3A00024297%21RIV11-MSM-22330___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC50 of 0.3 μg L-1 using a monoclonal antibody (Mab22F12) against BaP, similar t Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC50 of 0.3 μg L-1 using a monoclonal antibody (Mab22F12) against BaP, similar t
dcterms:title
Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food
skos:prefLabel
Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food
skos:notation
RIV/60461373:22330/10:00024297!RIV11-MSM-22330___
n4:aktivita
n16:P
n4:aktivity
P(7E08068)
n4:cisloPeriodika
1-2
n4:dodaniDat
n7:2011
n4:domaciTvurceVysledku
n15:1283243 n15:8580588
n4:druhVysledku
n13:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n8:predkladatel
n4:idSjednocenehoVysledku
251178
n4:idVysledku
RIV/60461373:22330/10:00024297
n4:jazykVysledku
n17:eng
n4:klicovaSlova
Polycyclic aromatic hydrocarbons; Flow cytometry; Persistent organic pollutants
n4:klicoveSlovo
n6:Persistent%20organic%20pollutants n6:Polycyclic%20aromatic%20hydrocarbons n6:Flow%20cytometry
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[1373F9DB9A64]
n4:nazevZdroje
Anal.Chim.Acta
n4:obor
n10:GM
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:projekt
n14:7E08068
n4:rokUplatneniVysledku
n7:2010
n4:svazekPeriodika
672
n4:tvurceVysledku
Pulkrabová, Jana Nielen, M. W. Hajšlová, Jana Meimaridou, A. Mintzas, D. Haasnoot, W. Noteboom, L.
n4:wos
000279851000003
s:issn
0003-2670
s:numberOfPages
6
n18:organizacniJednotka
22330