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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F10%3A00024237%21RIV11-MSM-22330___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Some time ago, we published an announcement that the azo group that closes model cyclic peptides is often reduced in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in the presence of 2,5-dihydroxybenzoic acid (2,5-DHB) as the matrix. In this work, we demonstrate that these peptides are ionized in all DHB matrix isomers, although threshold ionization laser energies as well as the reduction ratios differ in each matrix. Using a NALDI plate, we confirmed that their reduction depends on the presence of DHB matrix and that the hydrogen atoms participating in the reaction come from the DHB matrix hydroxyl group. We show that the reduction ratio is affected by the overall covalent structure of the peptide, by the presence of a free carboxyl group in DHB matrix, by the mutual position of the hydroxyl and carboxyl groups, as well as the laser beam intensity. Based on these results, it can be concluded that the azo-group reduction in cyclic peptides is a very complex process and we are Some time ago, we published an announcement that the azo group that closes model cyclic peptides is often reduced in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) in the presence of 2,5-dihydroxybenzoic acid (2,5-DHB) as the matrix. In this work, we demonstrate that these peptides are ionized in all DHB matrix isomers, although threshold ionization laser energies as well as the reduction ratios differ in each matrix. Using a NALDI plate, we confirmed that their reduction depends on the presence of DHB matrix and that the hydrogen atoms participating in the reaction come from the DHB matrix hydroxyl group. We show that the reduction ratio is affected by the overall covalent structure of the peptide, by the presence of a free carboxyl group in DHB matrix, by the mutual position of the hydroxyl and carboxyl groups, as well as the laser beam intensity. Based on these results, it can be concluded that the azo-group reduction in cyclic peptides is a very complex process and we are
dcterms:title
Azo-group reduction during matrix-assisted laser desorption/ionization process in the presence of 2,5-dyhydroxybenzoic acid Azo-group reduction during matrix-assisted laser desorption/ionization process in the presence of 2,5-dyhydroxybenzoic acid
skos:prefLabel
Azo-group reduction during matrix-assisted laser desorption/ionization process in the presence of 2,5-dyhydroxybenzoic acid Azo-group reduction during matrix-assisted laser desorption/ionization process in the presence of 2,5-dyhydroxybenzoic acid
skos:notation
RIV/60461373:22330/10:00024237!RIV11-MSM-22330___
n4:aktivita
n10:Z
n4:aktivity
Z(MSM6046137305)
n4:cisloPeriodika
22
n4:dodaniDat
n7:2011
n4:domaciTvurceVysledku
n8:9046976 n8:2544733
n4:druhVysledku
n17:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
248325
n4:idVysledku
RIV/60461373:22330/10:00024237
n4:jazykVysledku
n16:eng
n4:klicovaSlova
Flight Mass-Spectrometry; Desorption Ionisation MALDI; TOF MS Analysis; p-Aminophenol; Isomers; Identification; Peptide; Model
n4:klicoveSlovo
n9:Identification n9:Desorption%20Ionisation%20MALDI n9:Peptide n9:TOF%20MS%20Analysis n9:Isomers n9:Model n9:Flight%20Mass-Spectrometry n9:p-Aminophenol
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[349F4DEBBAF7]
n4:nazevZdroje
Rapid Communications in Mass Spectrometry
n4:obor
n14:CB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:rokUplatneniVysledku
n7:2010
n4:svazekPeriodika
24
n4:tvurceVysledku
Svoboda, Martin Kodíček, Milan
n4:wos
000284023400017
n4:zamer
n18:MSM6046137305
s:issn
0951-4198
s:numberOfPages
6
n15:organizacniJednotka
22330