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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F10%3A00023765%21RIV11-MPO-22330___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase. It is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents: 1-butanol, acetone and ethanol. The initiation of the solvents production coupled with endospore formation leads to decrease of the cell wall thickness. The thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method originated in Gram staining. The cell wall of G+ bacteria allows the entry of hexidium iodide (HI) and rhodamine 123 (RH123). However, the outer membrane of G- bacteria does not allow the uptake and therefore G+ bacteria are stained with higher fluorescence intensity than G- bacteria. The ratio of fluorescence intensity to forward scatter (FSC) was determined to correspond to G+ bacteria when clostridia were producing less of solvents. The significant drop of the ratio of fluorescence intensity to FSC to the level co Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase. It is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents: 1-butanol, acetone and ethanol. The initiation of the solvents production coupled with endospore formation leads to decrease of the cell wall thickness. The thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method originated in Gram staining. The cell wall of G+ bacteria allows the entry of hexidium iodide (HI) and rhodamine 123 (RH123). However, the outer membrane of G- bacteria does not allow the uptake and therefore G+ bacteria are stained with higher fluorescence intensity than G- bacteria. The ratio of fluorescence intensity to forward scatter (FSC) was determined to correspond to G+ bacteria when clostridia were producing less of solvents. The significant drop of the ratio of fluorescence intensity to FSC to the level co
dcterms:title
Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum
skos:prefLabel
Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum
skos:notation
RIV/60461373:22330/10:00023765!RIV11-MPO-22330___
n3:aktivita
n10:Z n10:S n10:P
n3:aktivity
P(FR-TI1/218), P(QH81323), S, Z(MSM6046137305)
n3:cisloPeriodika
4
n3:dodaniDat
n15:2011
n3:domaciTvurceVysledku
n9:5970385 n9:2569868 n9:5479886 n9:3943305 n9:6816517 n9:5377021 n9:4521137
n3:druhVysledku
n17:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
253945
n3:idVysledku
RIV/60461373:22330/10:00023765
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Flow cytometry; Gram staining; Clostridium pasteurianum; 1-butanol
n3:klicoveSlovo
n16:Flow%20cytometry n16:Gram%20staining n16:1-butanol n16:Clostridium%20pasteurianum
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[EBCEB5D698A8]
n3:nazevZdroje
Folia Microbiologica
n3:obor
n12:EI
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
7
n3:projekt
n8:FR-TI1%2F218 n8:QH81323
n3:rokUplatneniVysledku
n15:2010
n3:svazekPeriodika
55
n3:tvurceVysledku
Melzoch, Karel Lipovský, Jakub Linhová, Michaela Paulová, Leona Fribert, Petr Rychtera, Mojmír Patáková, Petra
n3:wos
000281277900009
n3:zamer
n13:MSM6046137305
s:issn
0015-5632
s:numberOfPages
5
n7:organizacniJednotka
22330