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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F09%3A00022416%21RIV10-MSM-22330___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation. Following isolation of DNA from the sample, a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively, was performed. The PCR solution and carbon particles conjugated with avidin are directly added to the nitrocellulose membrane with two test lines of immobilised antibodies specific for digoxigenin and fluorescein. The appearance of a black line indicates the presence of specific amplicon. We would like to present the NALFIA for the simultaneous detection of L. monocytogenes in particular and the genus Listeria in general, in food. Bacteria from the genus Listeria frequently contaminate a large variety of foods. Occurrence of Listeria strains in food may indicate errors in good hygienic and manufacturing practice, only L. monocytogenes is a significant human and animal
dcterms:title
Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food
skos:prefLabel
Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food
skos:notation
RIV/60461373:22330/09:00022416!RIV10-MSM-22330___
n3:aktivita
n11:P n11:Z
n3:aktivity
P(2B08050), Z(MSM6046137305)
n3:cisloPeriodika
27
n3:dodaniDat
n8:2010
n3:domaciTvurceVysledku
n16:8065462 n16:6725759 n16:4819640
n3:druhVysledku
n4:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
330179
n3:idVysledku
RIV/60461373:22330/09:00022416
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Listeria monocytogenes; Nucleic acid lateral flow immunoassay; DNA
n3:klicoveSlovo
n5:Nucleic%20acid%20lateral%20flow%20immunoassay n5:DNA n5:Listeria%20monocytogenes
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[48310B6E7189]
n3:nazevZdroje
Czech Journal of Food Sciences
n3:obor
n15:GM
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n6:2B08050
n3:rokUplatneniVysledku
n8:2009
n3:tvurceVysledku
Fukal, Ladislav Van Amerongen, Aart Wichers, Jan H. Blažková, Martina Rauch, Pavel Koets, Marjo
n3:wos
000269005600111
n3:zamer
n18:MSM6046137305
s:issn
1212-1800
s:numberOfPages
4
n9:organizacniJednotka
22330