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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F09%3A00022398%21RIV10-MSM-22330___
rdf:type
n19:Vysledek skos:Concept
dcterms:description
We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with two labelled primer sets, one generic and directed to a specific sequence of the gene encoding 16S rRNA from Listeria spp. and the other specific and directed to a part of the prfA gene encoding the central virulence gene regulator from the food pathogen Listeria monocytogenes (3.5 h). The PCR solution is directly added to the one-step assay device and the appearance of a grey/black line is indicative of the presence of specific amplicons (max 15 min). In all tests performed, the method correctly identified L. monocytogenes and strains of Listeria spp. PCR material of over 20 food samples was tested by NALFIA. The method proved to be useful for the detection of L. monocytogenes in different kinds of food samples. We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment of sample in Half Fraser broth (24 h). Following isolation of DNA, a duplex PCR is performed with two labelled primer sets, one generic and directed to a specific sequence of the gene encoding 16S rRNA from Listeria spp. and the other specific and directed to a part of the prfA gene encoding the central virulence gene regulator from the food pathogen Listeria monocytogenes (3.5 h). The PCR solution is directly added to the one-step assay device and the appearance of a grey/black line is indicative of the presence of specific amplicons (max 15 min). In all tests performed, the method correctly identified L. monocytogenes and strains of Listeria spp. PCR material of over 20 food samples was tested by NALFIA. The method proved to be useful for the detection of L. monocytogenes in different kinds of food samples.
dcterms:title
Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food
skos:prefLabel
Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food Development of a nucleic acid lateral flow immunoassay for simultaneous detection of Listeria spp. and Listeria monocytogenes in food
skos:notation
RIV/60461373:22330/09:00022398!RIV10-MSM-22330___
n3:aktivita
n13:P n13:Z
n3:aktivity
P(2B08050), Z(MSM6046137305)
n3:cisloPeriodika
6
n3:dodaniDat
n4:2010
n3:domaciTvurceVysledku
n15:6725759 n15:8065462
n3:druhVysledku
n17:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
310031
n3:idVysledku
RIV/60461373:22330/09:00022398
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Food-borne pathogen; Immunochromatographic test; Nucleic acid lateral flow immunoassay (NALFIA); Listeria monocytogenes; Food; Nucleic acid testing; Nanoparticles
n3:klicoveSlovo
n12:Food-borne%20pathogen n12:Listeria%20monocytogenes n12:Nucleic%20acid%20lateral%20flow%20immunoassay%20%28NALFIA%29 n12:Nucleic%20acid%20testing n12:Immunochromatographic%20test n12:Food n12:Nanoparticles
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[0DA900FEFBEF]
n3:nazevZdroje
European Food Research and Technology
n3:obor
n14:GM
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n11:2B08050
n3:rokUplatneniVysledku
n4:2009
n3:svazekPeriodika
229
n3:tvurceVysledku
Rauch, Pavel Van Amerongen, Aart Blažková, Martina Koets, Marjo
n3:wos
000270170000004
n3:zamer
n7:MSM6046137305
s:issn
1438-2377
s:numberOfPages
8
n9:organizacniJednotka
22330