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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F09%3A00022275%21RIV10-MSM-22330___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
Enterococci were presented in all tested samples of raw cow milk (six samples) at the level 103 ? 105 CFU ml-1, fresh cheeses (five samples) at the level 102 ? 106 CFU g-1 and semi-hard cheeses (five samples) at the level 103 ? 105 CFU g-1. All 33 isolated Enterococcus strains were screened for decarboxylase activity by usage differential growth medium and 20 of them possessed tyrosine decarboxylase activity. A collection of eight strains with the strongest decarboxylase activity were identified by species specific PCR as E. faecium (Z3, Z4, Br4 and 6/4D strains) and E. faecalis (Ž4, 3/3C and 4/1A strains). Enterococcus spp. Z1 strain was not identified at the species level by used methods, but the genus was confirmed by PCR method. Their tyrosin decarboxylase activity was confirmed by TLC and detection of tdc gene. Z1, Z3 and Z4 strains showed also histidine decarboxylase activity on the differential growth medium with histidine, but this activity was evaluated by TLC as a false positive reaction of Enterococci were presented in all tested samples of raw cow milk (six samples) at the level 103 ? 105 CFU ml-1, fresh cheeses (five samples) at the level 102 ? 106 CFU g-1 and semi-hard cheeses (five samples) at the level 103 ? 105 CFU g-1. All 33 isolated Enterococcus strains were screened for decarboxylase activity by usage differential growth medium and 20 of them possessed tyrosine decarboxylase activity. A collection of eight strains with the strongest decarboxylase activity were identified by species specific PCR as E. faecium (Z3, Z4, Br4 and 6/4D strains) and E. faecalis (Ž4, 3/3C and 4/1A strains). Enterococcus spp. Z1 strain was not identified at the species level by used methods, but the genus was confirmed by PCR method. Their tyrosin decarboxylase activity was confirmed by TLC and detection of tdc gene. Z1, Z3 and Z4 strains showed also histidine decarboxylase activity on the differential growth medium with histidine, but this activity was evaluated by TLC as a false positive reaction of
dcterms:title
Production of Biogenic Amines by Enterococci Production of Biogenic Amines by Enterococci
skos:prefLabel
Production of Biogenic Amines by Enterococci Production of Biogenic Amines by Enterococci
skos:notation
RIV/60461373:22330/09:00022275!RIV10-MSM-22330___
n3:aktivita
n15:P n15:Z
n3:aktivity
P(2B06048), Z(MSM6046137305)
n3:cisloPeriodika
6
n3:dodaniDat
n7:2010
n3:domaciTvurceVysledku
n5:7806876 n5:6900593 n5:5617766 n5:7902387
n3:druhVysledku
n16:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
336756
n3:idVysledku
RIV/60461373:22330/09:00022275
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Enterococcus; decarboxylase; tyrosine; histidine; TLC; PCR
n3:klicoveSlovo
n10:Enterococcus n10:TLC n10:PCR n10:histidine n10:decarboxylase n10:tyrosine
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[896CCF717313]
n3:nazevZdroje
Czech Journal of Food Science
n3:obor
n13:GM
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n11:2B06048
n3:rokUplatneniVysledku
n7:2009
n3:svazekPeriodika
27
n3:tvurceVysledku
Plocková, Milada Kučerová, Kateřina Tůma, Štěpán Ondráčková, Iva Svodová, Hana
n3:wos
000274102900009
n3:zamer
n4:MSM6046137305
s:issn
1212-1800
s:numberOfPages
6
n6:organizacniJednotka
22330