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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F09%3A00022152%21RIV10-MSM-22330___
rdf:type
n3:Vysledek skos:Concept
dcterms:description
The addition of proteinaceous binders to mortars and plasters has a long tradition. The protein additions were identified in many sacral and secular historical buildings. For this method of peptide mass mapping, three model mortar samples with protein additives were prepared. These samples were analysed fresh (1-2 weeks old) and after 9 months of natural ageing. The optimal duration of tryptic cleavage (2 h) and the lowest amount of material needed for relevant analysis of fresh and weathered samples were found: the sufficient amounts of weathered and fresh mortars were set to 0.05 and 0.005 g. The list of main tryptic peptides coming from milk additives (bovine milk, curd, and whey), their relative intensities and theoretical amino acid sequences assignment is presented. Several sequences have been %22de novo%22 confirmed by mass spectrometry. The addition of proteinaceous binders to mortars and plasters has a long tradition. The protein additions were identified in many sacral and secular historical buildings. For this method of peptide mass mapping, three model mortar samples with protein additives were prepared. These samples were analysed fresh (1-2 weeks old) and after 9 months of natural ageing. The optimal duration of tryptic cleavage (2 h) and the lowest amount of material needed for relevant analysis of fresh and weathered samples were found: the sufficient amounts of weathered and fresh mortars were set to 0.05 and 0.005 g. The list of main tryptic peptides coming from milk additives (bovine milk, curd, and whey), their relative intensities and theoretical amino acid sequences assignment is presented. Several sequences have been %22de novo%22 confirmed by mass spectrometry.
dcterms:title
Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials
skos:prefLabel
Towards proteomic analysis of milk proteins in historical building materials Towards proteomic analysis of milk proteins in historical building materials
skos:notation
RIV/60461373:22330/09:00022152!RIV10-MSM-22330___
n5:aktivita
n9:P n9:Z
n5:aktivity
P(GP203/07/P360), Z(MSM6046137305)
n5:cisloPeriodika
1-3
n5:dodaniDat
n8:2010
n5:domaciTvurceVysledku
n11:9046976 n11:6936903 n11:9697160 n11:8968136 n11:9374906
n5:druhVysledku
n18:J
n5:duvernostUdaju
n12:S
n5:entitaPredkladatele
n7:predkladatel
n5:idSjednocenehoVysledku
346579
n5:idVysledku
RIV/60461373:22330/09:00022152
n5:jazykVysledku
n19:eng
n5:klicovaSlova
Peptide mass mapping; Database; MALDI-TOF MS; Model mortar sample; Protein additive
n5:klicoveSlovo
n6:MALDI-TOF%20MS n6:Peptide%20mass%20mapping n6:Protein%20additive n6:Database n6:Model%20mortar%20sample
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[151C9403ABB8]
n5:nazevZdroje
International Journal of Mass Spectrometry
n5:obor
n17:CB
n5:pocetDomacichTvurcuVysledku
5
n5:pocetTvurcuVysledku
6
n5:projekt
n10:GP203%2F07%2FP360
n5:rokUplatneniVysledku
n8:2009
n5:svazekPeriodika
284
n5:tvurceVysledku
Kodíček, Milan Hnízda, Aleš Hynek, Radovan Kučková, Štěpánka Vaňková, Lucie Crhová, Michaela
n5:wos
000268059100006
n5:zamer
n15:MSM6046137305
s:issn
1387-3806
s:numberOfPages
5
n16:organizacniJednotka
22330