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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F09%3A00022120%21RIV10-MSM-22330___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the %22Rous-like%22 virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the %22HIV-like%22 virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the %22HIV-like%22, which retained fractional infectivity. Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) by mutating the frameshift sites to imitate the polyprotein organization of Rous sarcoma virus (Gag-Pro and Gag-Pro-Pol) or Human immunodeficiency virus (Gag and Gag-Pro-Pol). For the %22Rous-like%22 virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the %22HIV-like%22 virus an altered morphogenesis was observed. A mutant expressing Gag and Gag-Pro polyproteins and lacking Gag-Pro-Pol assembled intracellular particles at a level similar to the wild-type. Gag-Pro-Pol polyprotein alone neither formed immature particles nor processed the precursor. All the mutants were non-infectious except the %22HIV-like%22, which retained fractional infectivity.
dcterms:title
The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus.
skos:prefLabel
The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus. The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus.
skos:notation
RIV/60461373:22330/09:00022120!RIV10-MSM-22330___
n3:aktivita
n19:Z n19:P
n3:aktivity
P(1M0508), P(1M0520), P(GESCO/06/E001), P(KAN200100801), P(KAN208240651), P(ME 904), Z(AV0Z40550506), Z(MSM6046137305)
n3:cisloPeriodika
384
n3:dodaniDat
n15:2010
n3:domaciTvurceVysledku
n13:9372040 n13:5427975 n13:8571635
n3:druhVysledku
n4:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
318568
n3:idVysledku
RIV/60461373:22330/09:00022120
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Ribosomal frameshift; Retrovirus; Assembly; Mason-Pfizer monkey virus; Capsid
n3:klicoveSlovo
n7:Mason-Pfizer%20monkey%20virus n7:Ribosomal%20frameshift n7:Retrovirus n7:Assembly n7:Capsid
n3:kodStatuVydavatele
BE - Belgické království
n3:kontrolniKodProRIV
[0C3C146AAE44]
n3:nazevZdroje
Virology
n3:obor
n18:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
7
n3:projekt
n9:ME%20904 n9:1M0520 n9:1M0508 n9:KAN200100801 n9:KAN208240651 n9:GESCO%2F06%2FE001
n3:rokUplatneniVysledku
n15:2009
n3:tvurceVysledku
Sakalian, Michael Andreánský, Martin Rumlová, Michaela Ruml, Tomáš Hunter, Eric Kohoutová roz. Smékalová, Zdena Pichová, Iva
n3:wos
000263039200010
n3:zamer
n11:AV0Z40550506 n11:MSM6046137305
s:issn
0042-6822
s:numberOfPages
10
n14:organizacniJednotka
22330