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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F08%3A00021360%21RIV10-MSM-22330___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Tobacco seedlings (Nicotiana tabacum var. xanthi) were treated for 24 h with mono-(2- and 3-CBA), di-(2,5- and 3,4-CBA), and tri-(2,4,6- and 2,3,5-CBA)-chlorobenzoic acids (CBAs) and with the mixture of polychlorinated biphenyls - Delor 103, or cultivated for 1 or 2 weeks in soil polluted with the CBAs. DNA damage in nuclei of leaves and roots was evaluated by the comet assay. A significant increase in DNA damage was observed only at concentrations of CBAs that caused withering of leaves or had lethal effects within 2-4 weeks after the treatments. As the application of CBAs did not induce somatic mutations, the induced DNA migration is probably caused by necrotic DNA fragmentation and not by DNA damage resulting in genetic alteration. In contrast, the application of the monofunctional alkylating agent ethyl methanesulphonate as a positive control resulted in a dose-response increase of DNA damage and an increase of somatic mutations. Thus. the EMS-produced DNA migration is probably associated with gen Tobacco seedlings (Nicotiana tabacum var. xanthi) were treated for 24 h with mono-(2- and 3-CBA), di-(2,5- and 3,4-CBA), and tri-(2,4,6- and 2,3,5-CBA)-chlorobenzoic acids (CBAs) and with the mixture of polychlorinated biphenyls - Delor 103, or cultivated for 1 or 2 weeks in soil polluted with the CBAs. DNA damage in nuclei of leaves and roots was evaluated by the comet assay. A significant increase in DNA damage was observed only at concentrations of CBAs that caused withering of leaves or had lethal effects within 2-4 weeks after the treatments. As the application of CBAs did not induce somatic mutations, the induced DNA migration is probably caused by necrotic DNA fragmentation and not by DNA damage resulting in genetic alteration. In contrast, the application of the monofunctional alkylating agent ethyl methanesulphonate as a positive control resulted in a dose-response increase of DNA damage and an increase of somatic mutations. Thus. the EMS-produced DNA migration is probably associated with gen
dcterms:title
Genomic damage induced in tobacco plants by chlorobenzoic acids-Metabolic products of polychlorinated biphenyls Genomic damage induced in tobacco plants by chlorobenzoic acids-Metabolic products of polychlorinated biphenyls
skos:prefLabel
Genomic damage induced in tobacco plants by chlorobenzoic acids-Metabolic products of polychlorinated biphenyls Genomic damage induced in tobacco plants by chlorobenzoic acids-Metabolic products of polychlorinated biphenyls
skos:notation
RIV/60461373:22330/08:00021360!RIV10-MSM-22330___
n3:aktivita
n11:P n11:Z
n3:aktivity
P(GA203/06/0563), P(GA521/05/0500), Z(AV0Z50380511), Z(MSM6046137305)
n3:cisloPeriodika
2
n3:dodaniDat
n15:2010
n3:domaciTvurceVysledku
n9:9093095 n9:2752794
n3:druhVysledku
n7:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
369172
n3:idVysledku
RIV/60461373:22330/08:00021360
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Comet assay; Delor 103; Ethyl methanesulphonate; Nicotiana tabacum L. var. xanthi; Somatic mutations
n3:klicoveSlovo
n5:Nicotiana%20tabacum%20L.%20var.%20xanthi n5:Comet%20assay n5:Ethyl%20methanesulphonate n5:Somatic%20mutations n5:Delor%20103
n3:kodStatuVydavatele
BE - Belgické království
n3:kontrolniKodProRIV
[8F5D26CEAF16]
n3:nazevZdroje
Mutation Research/Genetic Toxicology and Environmental Mutagenesis
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n6:GA521%2F05%2F0500 n6:GA203%2F06%2F0563
n3:rokUplatneniVysledku
n15:2008
n3:svazekPeriodika
657
n3:tvurceVysledku
Lovecká, Petra Gicner, Tomáš Vrchotová, Blanka
n3:wos
000261851000009
n3:zamer
n8:AV0Z50380511 n8:MSM6046137305
s:issn
1383-5718
s:numberOfPages
5
n13:organizacniJednotka
22330