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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F08%3A00019963%21RIV09-MSM-22330___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Immunoaffinity chromatography (IAC) or immunoextraction (IE) is based on a highly selective and affinitive interaction between antibodies immobilized on a solid support and analytes-antigens present in a sample. Thus enabling to concentrate and clean-up the analytes from complex biological matrixes, such as plant extracts. Isoflavonoids belong to secondary plant metabolites and some of them are known for their pharmacological activities such as estrogenic, antiviral, antibacterial, antioxidative etc. Legumes are known as their main source. Nevertheless, the presence of isoflavonoids has been reported in about sixty other families. In non-legumes, isoflavonoids are present in lower concentrations, which results in a more difficult detection and identification. This was the reason for the development of an off-line combination of IAC and HPLC-MS for the detection of isoflavonoids in non-legume plant extracts. Immunosorbents were prepared by immobilization of polyclonal rabbit antibodies against con Immunoaffinity chromatography (IAC) or immunoextraction (IE) is based on a highly selective and affinitive interaction between antibodies immobilized on a solid support and analytes-antigens present in a sample. Thus enabling to concentrate and clean-up the analytes from complex biological matrixes, such as plant extracts. Isoflavonoids belong to secondary plant metabolites and some of them are known for their pharmacological activities such as estrogenic, antiviral, antibacterial, antioxidative etc. Legumes are known as their main source. Nevertheless, the presence of isoflavonoids has been reported in about sixty other families. In non-legumes, isoflavonoids are present in lower concentrations, which results in a more difficult detection and identification. This was the reason for the development of an off-line combination of IAC and HPLC-MS for the detection of isoflavonoids in non-legume plant extracts. Immunosorbents were prepared by immobilization of polyclonal rabbit antibodies against con Isoflavonoidy (3-fenylchromony) jsou sekundární metabolity rostlin zajímavé z hlediska svých biologických účinků. Nejvýznamnějším zdrojem isoflavonoidů jsou bobovité rostliny (Leguminosae), ale přítomnost těchto látek byla zjištěna v řadě dalších taxonů1,2. Mimo čeleď Leguminosae jsou zastoupeny v nižších koncentracích, což znesnadňuje jejich detekci a identifikaci. Proto bylo k zakoncentrování a přečištění rostlinných extraktů před analýzou pomocí HPLC-MS navrženo použití imunoafinitních sorbentů. Imunoafinitní sorbenty byly připraveny imobilizací polyklonálních králičích protilátek proti vybraným isoflavonoidům (biochanin A, genistein, daidzein) na pevný nosič Affi-Gel 10 (Bio-Rad laboatories)3. Kapacita stanovená vyhodnocením kompetice standardu příslušného isoflavonoidu s jeho 125I značeným analogem dosahovala u jednotlivých sorbentů hodnot řádu desetin až jednotek mikrogramů analytu na militr gelu. Byly zjistěny optimální podmínky eluce a vyvinut postup imunoafinitní extrakce. Připravené sorbent
dcterms:title
Immunoaffinity sorbents for SPE purification of isoflavonoids Immunoaffinity sorbents for SPE purification of isoflavonoids Imunoafinitní sorbenty pro SPE isoflavonoidů
skos:prefLabel
Immunoaffinity sorbents for SPE purification of isoflavonoids Imunoafinitní sorbenty pro SPE isoflavonoidů Immunoaffinity sorbents for SPE purification of isoflavonoids
skos:notation
RIV/60461373:22330/08:00019963!RIV09-MSM-22330___
n3:aktivita
n12:Z n12:P
n3:aktivity
P(GA525/06/0864), Z(MSM6046137305)
n3:dodaniDat
n9:2009
n3:domaciTvurceVysledku
n13:1056379
n3:druhVysledku
n18:D
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
371447
n3:idVysledku
RIV/60461373:22330/08:00019963
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Isoflavone; IAC; SPE; HPLC
n3:klicoveSlovo
n5:HPLC n5:Isoflavone n5:SPE n5:IAC
n3:kontrolniKodProRIV
[3D1FB859C197]
n3:mistoKonaniAkce
Salamanca, Španělsko
n3:mistoVydani
Salamanca
n3:nazevZdroje
Polyphenols Communications
n3:obor
n16:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n8:GA525%2F06%2F0864
n3:rokUplatneniVysledku
n9:2008
n3:tvurceVysledku
Lanková, Petra Prokudina, Elena Lapčík, Oldřich
n3:typAkce
n17:WRD
n3:zahajeniAkce
2008-06-08+02:00
n3:zamer
n11:MSM6046137305
s:numberOfPages
2
n21:hasPublisher
University of Salamanca
n22:isbn
978-84-691-4333-9
n20:organizacniJednotka
22330