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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F07%3A00019275%21RIV08-MSM-22330___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and high con Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and high con Frakcionace specií Co, Cu, Fe, Mn, Mo, Ni, P a Zn v extraktech pohankové krupice a amarantové mouky v 0,02 mol/l Tris-HCL (pH=7,5) byla provedena metodou SEC/ICP-MS.
dcterms:title
Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS Analýza frakcí prvkových specií v pohankové a amarantové mouce metodami SEC/ICP-MS a MALDI-MS Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS
skos:prefLabel
Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS Analýza frakcí prvkových specií v pohankové a amarantové mouce metodami SEC/ICP-MS a MALDI-MS Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS
skos:notation
RIV/60461373:22330/07:00019275!RIV08-MSM-22330___
n5:strany
895-904
n5:aktivita
n14:Z
n5:aktivity
Z(MSM6046137305)
n5:cisloPeriodika
5-6
n5:dodaniDat
n8:2008
n5:domaciTvurceVysledku
n7:1250558 n7:8440646 n7:9046976 n7:8995354 n7:2901153 n7:1120530
n5:druhVysledku
n12:J
n5:duvernostUdaju
n13:S
n5:entitaPredkladatele
n11:predkladatel
n5:idSjednocenehoVysledku
409461
n5:idVysledku
RIV/60461373:22330/07:00019275
n5:jazykVysledku
n10:eng
n5:klicovaSlova
Amaranth; buckwheat; speciation; trace elements; MALDI-MS; ICP-MS
n5:klicoveSlovo
n6:speciation n6:buckwheat n6:Amaranth n6:MALDI-MS n6:ICP-MS n6:trace%20elements
n5:kodStatuVydavatele
DE - Spolková republika Německo
n5:kontrolniKodProRIV
[26AAE12DF124]
n5:nazevZdroje
European Food Research and Technology
n5:obor
n18:GM
n5:pocetDomacichTvurcuVysledku
6
n5:pocetTvurcuVysledku
7
n5:rokUplatneniVysledku
n8:2007
n5:svazekPeriodika
225
n5:tvurceVysledku
Šantrůček, Jiří Komínková, Jana Polák, Jan Kodíček, Milan Mestek, Oto Koplík, Richard Kvasnička, František
n5:zamer
n17:MSM6046137305
s:issn
1438-2377
s:numberOfPages
10
n16:organizacniJednotka
22330