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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F06%3A00017798%21RIV07-MSM-22330___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. All above mentioned techniques have been optimized to be able to reach special electrophoresis pattern and discriminate among different species. RAPD protocol was optimized by using two different primers which both have been found to be effective for the RAPD fingerprinting of assessed bacilli. Amplification of the 16S-23S rRNA ITS produced multiple bands in gel electrophoresis, implying multiple copies of variable length in the ITS of these bacteria. The ITS groupings were easier to interpret than the RAPD types and hence ITS typing may be mo Testy, založené na metodách molekulární biologie, se používají k identifikaci termofilních a mezofilních bacilů. Pět termofilních izolátů rodu Bacillus a tři mezofilní izoláty rodu Bacillus bylo podrobeno genotypizaci s využitím náhodně pomnožené polymorfické DNA (RAPD), s využitím amplifikace 16S-23S r RNA intergenového transkripčního spaceru (ITS-PCR), s využitím polymorfismu délky restrikčních fragmentů (RFLP) 16S r RNA a fragmentů ITS-PCR (ITS-PCR RFLP). Všechny uvedené metody byly optimalizovány tak, aby byly získány elektroforetogramy, z nichž bylo možné podle specifického vzoru rozlišit mezi jednotlivými druhy. RAPD protokol byl optimalizován s použitím dvou různých primerů, které oba se ukázaly jako vhodné pro RAPD fingerprinting. Po amplifikaci 16S-23S r RNA ITS bylo získáno po elektroforéze několik bandů, což vypovídá o několika kopiích ITS rozdílné délky u těchto bakterií. Vzory ITS bandů bylo snazší vyhodnotit než vzory RAPD, a proto se pro praxi ukázala jako vhodnější metoda ITS-PCR. V Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. All above mentioned techniques have been optimized to be able to reach special electrophoresis pattern and discriminate among different species. RAPD protocol was optimized by using two different primers which both have been found to be effective for the RAPD fingerprinting of assessed bacilli. Amplification of the 16S-23S rRNA ITS produced multiple bands in gel electrophoresis, implying multiple copies of variable length in the ITS of these bacteria. The ITS groupings were easier to interpret than the RAPD types and hence ITS typing may be mo
dcterms:title
Molecular characterization of thermophilic and mesophilic Bacillus species Molecular characterization of thermophilic and mesophilic Bacillus species Molekulární charakterizace termofilních a mezofilních druhů rodu Bacillus
skos:prefLabel
Molecular characterization of thermophilic and mesophilic Bacillus species Molecular characterization of thermophilic and mesophilic Bacillus species Molekulární charakterizace termofilních a mezofilních druhů rodu Bacillus
skos:notation
RIV/60461373:22330/06:00017798!RIV07-MSM-22330___
n3:strany
27-30
n3:aktivita
n15:Z
n3:aktivity
Z(MSM6046137305)
n3:cisloPeriodika
1
n3:dodaniDat
n6:2007
n3:domaciTvurceVysledku
n4:4521137 n4:1113992 n4:3943305
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
486600
n3:idVysledku
RIV/60461373:22330/06:00017798
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Thermophilic Bacillus; Mesophilic Bacillus; PCR; RAPD; RFLP; ITS-PCR; ITS-PCR RFLP
n3:klicoveSlovo
n5:PCR n5:RAPD n5:Thermophilic%20Bacillus n5:RFLP n5:Mesophilic%20Bacillus n5:ITS-PCR n5:ITS-PCR%20RFLP
n3:kodStatuVydavatele
GR - Řecká republika
n3:kontrolniKodProRIV
[5A929C48A13D]
n3:nazevZdroje
WSEAS Transactions on Biology and Biomedicine
n3:obor
n10:EI
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n6:2006
n3:svazekPeriodika
3
n3:tvurceVysledku
Melzoch, Karel Piterková, Lucie Skarkeová, Martina Patáková, Petra
n3:zamer
n12:MSM6046137305
s:issn
1109-9518
s:numberOfPages
4
n14:organizacniJednotka
22330