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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F05%3A00022532%21RIV10-MSM-22330___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
In the present work, the comparison study of enzyme-linked immunosorbent assays (ELISAs) with colorimetric detection and ELISAs with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb is described. Both conjugate-coated formats are based on identical monoclonal antibodies and homologous protein conjugates. The sensitivity of immunochemical method was expressed as a detection limit, a linear working range and an I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent technique to detect lower concentration of HRP allowed to decreased the optimal antibody and conjugate concentrations and thus to reach lower analytical parameters. The experimental comparison of the analytical performance of ELISAs was carried out by simultaneous analysis of apple-strawberry baby food extracts spiked with mentioned pesticides at three different concentration levels. The extracts were prepared in two duplicate sets - crude extracts and extracts purified by In the present work, the comparison study of enzyme-linked immunosorbent assays (ELISAs) with colorimetric detection and ELISAs with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb is described. Both conjugate-coated formats are based on identical monoclonal antibodies and homologous protein conjugates. The sensitivity of immunochemical method was expressed as a detection limit, a linear working range and an I50 value. In comparison with colorimetric ELISA, the ability of the chemiluminescent technique to detect lower concentration of HRP allowed to decreased the optimal antibody and conjugate concentrations and thus to reach lower analytical parameters. The experimental comparison of the analytical performance of ELISAs was carried out by simultaneous analysis of apple-strawberry baby food extracts spiked with mentioned pesticides at three different concentration levels. The extracts were prepared in two duplicate sets - crude extracts and extracts purified by
dcterms:title
Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides.
skos:prefLabel
Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides.
skos:notation
RIV/60461373:22330/05:00022532!RIV10-MSM-22330___
n3:aktivita
n5:S
n3:aktivity
S
n3:cisloPeriodika
528
n3:dodaniDat
n8:2010
n3:domaciTvurceVysledku
n4:8065462 n4:2217481 n4:2880199
n3:druhVysledku
n16:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
512175
n3:idVysledku
RIV/60461373:22330/05:00022532
n3:jazykVysledku
n14:eng
n3:klicovaSlova
N-methylcarbamate insecticides; baby food; ELISA; HPLC/MS/MS; colorimetric detection; chemiluminescent detection
n3:klicoveSlovo
n6:ELISA n6:N-methylcarbamate%20insecticides n6:HPLC%2FMS%2FMS n6:colorimetric%20detection n6:baby%20food n6:chemiluminescent%20detection
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[2633597FA011]
n3:nazevZdroje
Anal.Chim.Acta
n3:obor
n17:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
9
n3:rokUplatneniVysledku
n8:2005
n3:svazekPeriodika
neuveden
n3:tvurceVysledku
Kovalczuk, Tomáš Girotti, Stephano Montoya, Angel Moreno, Maria-Jose Fini, Fabiana Rauch, Pavel Abad, Antonio Ferri, Elida Holubová, Roz.mičková, Barbora
n3:wos
000226455800013
s:issn
0003-2670
s:numberOfPages
6
n9:organizacniJednotka
22330