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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F05%3A00016398%21RIV06-GA0-22330___
rdf:type
skos:Concept n7:Vysledek
dcterms:description
In contrast to other retroviruses as HIV, Mason-Pfizer monkey virus (M-PMV) preassembles immature capsids in the cytoplasm. We have performed the in vitro assembly of purified bacterially expressed proteins to address the difference of morphogenically distinct retroviruses M-PMV and HIV in their ability to assemble VLPs from minimized assembly competent domains. The fusion capsid and nucleocapsid protein (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of mature and immature particles, respectively. We found dramatically different ability to assemble CANC particles between these two viruses. Also ProCANC particles of M-PMV differed from that of HIV forming spheres and tubes, respectively. The requirements for factors affecting assembly were also evaluated and the amount of incorporated fluorescently labeled nucleic acids was determined. The presence of purified RNA efficiently promoted formation of ProCANC spherical particles. The oligonucleotides also supported In contrast to other retroviruses as HIV, Mason-Pfizer monkey virus (M-PMV) preassembles immature capsids in the cytoplasm. We have performed the in vitro assembly of purified bacterially expressed proteins to address the difference of morphogenically distinct retroviruses M-PMV and HIV in their ability to assemble VLPs from minimized assembly competent domains. The fusion capsid and nucleocapsid protein (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of mature and immature particles, respectively. We found dramatically different ability to assemble CANC particles between these two viruses. Also ProCANC particles of M-PMV differed from that of HIV forming spheres and tubes, respectively. The requirements for factors affecting assembly were also evaluated and the amount of incorporated fluorescently labeled nucleic acids was determined. The presence of purified RNA efficiently promoted formation of ProCANC spherical particles. The oligonucleotides also supported In contrast to other retroviruses as HIV, Mason-Pfizer monkey virus (M-PMV) preassembles immature capsids in the cytoplasm. We have performed the in vitro assembly of purified bacterially expressed proteins to address the difference of morphogenically distinct retroviruses M-PMV and HIV in their ability to assemble VLPs from minimized assembly competent domains. The fusion capsid and nucleocapsid protein (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of mature and immature particles, respectively. We found dramatically different ability to assemble CANC particles between these two viruses. Also ProCANC particles of M-PMV differed from that of HIV forming spheres and tubes, respectively. The requirements for factors affecting assembly were also evaluated and the amount of incorporated fluorescently labeled nucleic acids was determined. The presence of purified RNA efficiently promoted formation of ProCANC spherical particles. The oligonucleotides also supported
dcterms:title
In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids Studie in vitro skládání CA-NC kapsid Mason-Pfizerova opičího viru
skos:prefLabel
In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids Studie in vitro skládání CA-NC kapsid Mason-Pfizerova opičího viru In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids
skos:notation
RIV/60461373:22330/05:00016398!RIV06-GA0-22330___
n4:aktivita
n12:P
n4:aktivity
P(1M0520), P(GP203/03/P094)
n4:dodaniDat
n9:2006
n4:domaciTvurceVysledku
n5:6489419 n5:7091621 n5:9372040 n5:8571635
n4:druhVysledku
n10:O
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
524482
n4:idVysledku
RIV/60461373:22330/05:00016398
n4:jazykVysledku
n15:eng
n4:klicovaSlova
Mason-Pfizer monkey virus; in vitro assembly
n4:klicoveSlovo
n11:in%20vitro%20assembly n11:Mason-Pfizer%20monkey%20virus
n4:kontrolniKodProRIV
[C63306A19788]
n4:obor
n16:EE
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:projekt
n14:1M0520 n14:GP203%2F03%2FP094
n4:rokUplatneniVysledku
n9:2005
n4:tvurceVysledku
Haubová, Šárka Ruml, Tomáš Ulbrich, Pavel Rumlová, Michaela
n17:organizacniJednotka
22330