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Statements

Subject Item
n2:RIV%2F60461373%3A22330%2F00%3A00013228%21RIV06-MSM-22330___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Ke stanovení aktivity trypsin inhibitoru (TIA) byla použita metoda se syntetickým substrátem BAPNA (N--benzoyl-DL-arginin-p-nitroanilid). K extrakci bílkovin byl použit glycinový pufr pH 11 obsahující močovinu a EDTA, 2 h míchání a odstřeďování. Syntetický substrát BAPNA je hydrolyzován trypsinem a měří se absorbance žlutého p-nitroanilidu při 410 nm. Byly vyhodnoceny změny TIA během klíčení a následujícího mikrovlnného ohřevu a při konvenčním sušení naklíčeného hrachu do sušiny 12 - 14 %. The method by means synthetic substrate BAPNA (N--benzoyl-DL-arginin-p-nitroanilid) was used for trypsin-inhibitor activity (TIA) determination in pea seeds. To the extraction of proteins is used glycine buffer pH 11 containing urea and EDTA, 2 h stirring and centrifugation. Synthetic substrate BAPNA is hydrolysed by trypsin and the absorbance of dislodged yellow p-nitroanilid is measured at 410 nm. The changes of TIA during germination and the following microwave treatment and the conventional drying of germinated pea to the final moisture 12 - 14 % were evaluated. The method by means synthetic substrate BAPNA (N--benzoyl-DL-arginin-p-nitroanilid) was used for trypsin-inhibitor activity (TIA) determination in pea seeds. To the extraction of proteins is used glycine buffer pH 11 containing urea and EDTA, 2 h stirring and centrifugation. Synthetic substrate BAPNA is hydrolysed by trypsin and the absorbance of dislodged yellow p-nitroanilid is measured at 410 nm. The changes of TIA during germination and the following microwave treatment and the conventional drying of germinated pea to the final moisture 12 - 14 % were evaluated.
dcterms:title
Changes of trypsin inhibitor activity during microwave treatment of germinated pea Změna aktivityTrypsin-inhibitoru během mikrovlnného ohřevu naklíčeného hrachu Changes of trypsin inhibitor activity during microwave treatment of germinated pea
skos:prefLabel
Changes of trypsin inhibitor activity during microwave treatment of germinated pea Změna aktivityTrypsin-inhibitoru během mikrovlnného ohřevu naklíčeného hrachu Changes of trypsin inhibitor activity during microwave treatment of germinated pea
skos:notation
RIV/60461373:22330/00:00013228!RIV06-MSM-22330___
n3:strany
81-82
n3:aktivita
n15:Z n15:P
n3:aktivity
P(OK 325), Z(MSM 223300005)
n3:cisloPeriodika
18
n3:dodaniDat
n4:2006
n3:domaciTvurceVysledku
n5:8552363 n5:3321509
n3:druhVysledku
n18:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
706523
n3:idVysledku
RIV/60461373:22330/00:00013228
n3:jazykVysledku
n6:eng
n3:klicovaSlova
pea; germination; microwave treatment; trypsin-inhibitor activity
n3:klicoveSlovo
n12:trypsin-inhibitor%20activity n12:pea n12:germination n12:microwave%20treatment
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[BB9BC9A47809]
n3:nazevZdroje
Czech Journal of Food Science
n3:obor
n19:GM
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n13:OK%20325
n3:rokUplatneniVysledku
n4:2000
n3:tvurceVysledku
Skulinová, Michaela Kadlec, Pavel
n3:zamer
n10:MSM%20223300005
s:issn
1212-1800
s:numberOfPages
2
n8:organizacniJednotka
22330