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Statements

Subject Item
n2:RIV%2F60461373%3A22310%2F13%3A43895909%21RIV14-MPO-22310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Two types of cellulose-based materials, 6-carboxycellulose with 2.1 or 6.6 wt% of -COOH groups, were prepared and tested for potential use in tissue engineering. The materials were functionalized with arginine, i.e. an amino acid with a basic side chain, or with chitosan, in order to balance the relatively acid character of oxidized cellulose molecules, and were seeded with vascular smooth muscle cells (VSMC). The cell adhesion and growth were then evaluated directly on the materials, and also on the underlying polystyrene culture dishes. Of these two types of studied materials, 6-carboxycellulose with 2.1 wt% of -COOH groups was more appropriate for cell colonization. The cells on this material achieved an elongated shape, while they were spherical in shape on the other materials. The number of cells and the concentration (per mg of protein) of contractile proteins alpha-actin and SM1 and SM2 myosins, i.e. markers of the phenotypic maturation of VSMC, were also significantly higher on this material. Functionalization of the material with arginine and chitosan further improved the phenotypic maturation of VSMC. Chitosan also improved the adhesion and growth of these cells. In comparison with the control polystyrene dishes, the proliferation of cells on our cellulose-based materials was relatively low. This suggests that these materials can be used in applications where high proliferation activity of cells is not desirable, e.g. proliferation of VSMC on vascular prostheses. Alternatively, the cell proliferation might be enhanced by another more efficient modification, which would require further research. Two types of cellulose-based materials, 6-carboxycellulose with 2.1 or 6.6 wt% of -COOH groups, were prepared and tested for potential use in tissue engineering. The materials were functionalized with arginine, i.e. an amino acid with a basic side chain, or with chitosan, in order to balance the relatively acid character of oxidized cellulose molecules, and were seeded with vascular smooth muscle cells (VSMC). The cell adhesion and growth were then evaluated directly on the materials, and also on the underlying polystyrene culture dishes. Of these two types of studied materials, 6-carboxycellulose with 2.1 wt% of -COOH groups was more appropriate for cell colonization. The cells on this material achieved an elongated shape, while they were spherical in shape on the other materials. The number of cells and the concentration (per mg of protein) of contractile proteins alpha-actin and SM1 and SM2 myosins, i.e. markers of the phenotypic maturation of VSMC, were also significantly higher on this material. Functionalization of the material with arginine and chitosan further improved the phenotypic maturation of VSMC. Chitosan also improved the adhesion and growth of these cells. In comparison with the control polystyrene dishes, the proliferation of cells on our cellulose-based materials was relatively low. This suggests that these materials can be used in applications where high proliferation activity of cells is not desirable, e.g. proliferation of VSMC on vascular prostheses. Alternatively, the cell proliferation might be enhanced by another more efficient modification, which would require further research.
dcterms:title
Cellulose-based materials as scaffolds for tissue engineering Cellulose-based materials as scaffolds for tissue engineering
skos:prefLabel
Cellulose-based materials as scaffolds for tissue engineering Cellulose-based materials as scaffolds for tissue engineering
skos:notation
RIV/60461373:22310/13:43895909!RIV14-MPO-22310___
n17:predkladatel
n18:orjk%3A22310
n4:aktivita
n16:I n16:P n16:Z
n4:aktivity
I, P(2A-1TP1/073), P(GAP108/12/1168), Z(AV0Z50110509)
n4:cisloPeriodika
5
n4:dodaniDat
n15:2014
n4:domaciTvurceVysledku
n9:8957940 n9:5486831 n9:2029057
n4:druhVysledku
n14:J
n4:duvernostUdaju
n6:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
64494
n4:idVysledku
RIV/60461373:22310/13:43895909
n4:jazykVysledku
n13:eng
n4:klicovaSlova
Chitosan; Contractile phenotype; Cell differentiation; Vascular smooth muscle cells; Oxidized cellulose; Polysaccharide materials
n4:klicoveSlovo
n11:Chitosan n11:Cell%20differentiation n11:Polysaccharide%20materials n11:Oxidized%20cellulose n11:Contractile%20phenotype n11:Vascular%20smooth%20muscle%20cells
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[62F8024989B6]
n4:nazevZdroje
Cellulose
n4:obor
n19:JJ
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
8
n4:projekt
n5:2A-1TP1%2F073 n5:GAP108%2F12%2F1168
n4:rokUplatneniVysledku
n15:2013
n4:svazekPeriodika
20
n4:tvurceVysledku
Kolářová, Kateřina Novotná, Katarina Havelka, Pavel Švorčík, Václav Sopuch, Tomáš Bačáková, Lucie Vosmanská, Vladimíra Lisá, Věra
n4:wos
000324494200005
n4:zamer
n7:AV0Z50110509
s:issn
0969-0239
s:numberOfPages
16
n10:doi
10.1007/s10570-013-0006-4
n20:organizacniJednotka
22310