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Statements

Subject Item
n2:RIV%2F60460709%3A_____%2F04%3A01%3A_3621%21RIV06-MZE-60460709
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need for Acaroid mites are serious pests of stored products and households. They produce allergens and transmit mycotoxin-producing fungi. The infestation of stored food by mites strongly decreases food safety and endangers public health. There is urgent need forrapid detection methods such as ELISA. The aim of our work was therefore to prepare and characterise antibodies for detection of the flour mite (Acarus siro). Two polyclonal antibodies (anti- A.siroK53 and anti-A.siroK54) were prepared against an antigen in the form of protein fraction from whole body homogenates of Acarus siro. Specificity and sensitivity of the prepared antibodies were evaluated. We did not observe any cross-reactivity with stored beetles, moths, other mite species, micro-fungi, therearing diet of mites (yeast and wheat diet) and extracts from wheat kernels. Cross-reactivity to Acarus gracilis was found out. This phenomenon is discussed in relation to the evolution position of this species. The obtained antibodies (anti-A.siroK53 a
dcterms:title
Polyclonal antibodies pro detekci moucného cerva Acarus siro Polyclonal antibodies for detection of flour mite Acarus siro Polyclonal antibodies for detection of flour mite Acarus siro
skos:prefLabel
Polyclonal antibodies pro detekci moucného cerva Acarus siro Polyclonal antibodies for detection of flour mite Acarus siro Polyclonal antibodies for detection of flour mite Acarus siro
skos:notation
RIV/60460709:_____/04:01:_3621!RIV06-MZE-60460709
n3:strany
711-720
n3:aktivita
n15:P
n3:aktivity
P(QF4071)
n3:cisloPeriodika
03
n3:dodaniDat
n4:2006
n3:domaciTvurceVysledku
n17:2841495
n3:druhVysledku
n12:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
580173
n3:idVysledku
RIV/60460709:_____/04:01:_3621
n3:jazykVysledku
n16:eng
n3:klicovaSlova
ELISA, PCR, cereal products, food, monitoring, detection
n3:klicoveSlovo
n5:cereal%20products n5:PCR n5:ELISA n5:food n5:monitoring n5:detection
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[8A837ABAD046]
n3:nazevZdroje
Phytophaga 14
n3:obor
n11:GM
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n7:QF4071
n3:rokUplatneniVysledku
n4:2004
n3:svazekPeriodika
14
n3:tvurceVysledku
Ryšánek, Pavel Stejskal, Václav Chalupníková, Julie Kudlíková, Iva Hubert, Jan
s:numberOfPages
9