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Statements

Subject Item
n2:RIV%2F60460709%3A41340%2F14%3A64703%21RIV15-MZE-41340___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level. A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level.
dcterms:title
Comparative morphological and molecular identification of Haemonchus species in sheep Comparative morphological and molecular identification of Haemonchus species in sheep
skos:prefLabel
Comparative morphological and molecular identification of Haemonchus species in sheep Comparative morphological and molecular identification of Haemonchus species in sheep
skos:notation
RIV/60460709:41340/14:64703!RIV15-MZE-41340___
n6:aktivita
n14:P
n6:aktivity
P(QI111A199)
n6:cisloPeriodika
2
n6:dodaniDat
n13:2015
n6:domaciTvurceVysledku
n17:2809257
n6:druhVysledku
n10:J
n6:duvernostUdaju
n16:S
n6:entitaPredkladatele
n15:predkladatel
n6:idSjednocenehoVysledku
7948
n6:idVysledku
RIV/60460709:41340/14:64703
n6:jazykVysledku
n7:eng
n6:klicovaSlova
barber´s pole worm, spicule, discrimiant function, PCR-RFLP, sequencing, internal transcribed spacer
n6:klicoveSlovo
n8:sequencing n8:barber%C2%B4s%20pole%20worm n8:PCR-RFLP n8:internal%20transcribed%20spacer n8:discrimiant%20function n8:spicule
n6:kodStatuVydavatele
PL - Polská republika
n6:kontrolniKodProRIV
[0478C8CE77F5]
n6:nazevZdroje
Helminthologia
n6:obor
n18:GJ
n6:pocetDomacichTvurcuVysledku
1
n6:pocetTvurcuVysledku
9
n6:projekt
n9:QI111A199
n6:rokUplatneniVysledku
n13:2014
n6:svazekPeriodika
51
n6:tvurceVysledku
Vašek, Jakub Vadlejch, Jaroslav Vejl, Pavel Lukešová, Daniela Jankovská, Ivana Salaba, Ondřej Čadková, Zuzana Sedlák, Petr Langrová, Iva
n6:wos
000338495400008
s:issn
0440-6605
s:numberOfPages
11
n12:organizacniJednotka
41340