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Statements

Subject Item
n2:RIV%2F60460709%3A41320%2F07%3A17535%21RIV08-MSM-41320___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Somatic embryogenic cultures were established from proembryonal suspensor masses (PEMs) derived from mature seeds of Norway spruce. In this study we used more than 4,300 seeds of Picea abies from randomly collected commercial seed lot (originated from open-pollination). Most of the studies are focused on selected genotypes known for higher response to propagation protocols. As indicated in this study, there is a significant variation in success rate of somatic embryogenesis in randomly selected seed lot of Norway spruce. Nutrient GD and LP media and different level of plant grow regulators (BA, NAA, kinetin and 2,4D) were used for initiation and proliferation of embryogenic cultures. Transfer of embryogenic callus onto medium containing abscisic acid stimulated development of early-established individual embryos. Media GD and LP supplemented with ABA (7.5; 20; 38 μM) and PEG 4000 (2%), were used for stadium of maturation. Conversion of somatic embryos to plantlets was stimulated by partial desic Somatic embryogenic cultures were established from proembryonal suspensor masses (PEMs) derived from mature seeds of Norway spruce. In this study we used more than 4,300 seeds of Picea abies from randomly collected commercial seed lot (originated from open-pollination). Most of the studies are focused on selected genotypes known for higher response to propagation protocols. As indicated in this study, there is a significant variation in success rate of somatic embryogenesis in randomly selected seed lot of Norway spruce. Nutrient GD and LP media and different level of plant grow regulators (BA, NAA, kinetin and 2,4D) were used for initiation and proliferation of embryogenic cultures. Transfer of embryogenic callus onto medium containing abscisic acid stimulated development of early-established individual embryos. Media GD and LP supplemented with ABA (7.5; 20; 38 μM) and PEG 4000 (2%), were used for stadium of maturation. Conversion of somatic embryos to plantlets was stimulated by partial desic Somatic embryogenic cultures were established from proembryonal suspensor masses (PEMs) derived from mature seeds of Norway spruce. In this study we used more than 4,300 seeds of Picea abies from randomly collected commercial seed lot (originated from open-pollination). Most of the studies are focused on selected genotypes known for higher response to propagation protocols. As indicated in this study, there is a significant variation in success rate of somatic embryogenesis in randomly selected seed lot of Norway spruce. Nutrient GD and LP media and different level of plant grow regulators (BA, NAA, kinetin and 2,4D) were used for initiation and proliferation of embryogenic cultures. Transfer of embryogenic callus onto medium containing abscisic acid stimulated development of early-established individual embryos. Media GD and LP supplemented with ABA (7.5; 20; 38 μM) and PEG 4000 (2%), were used for stadium of maturation. Conversion of somatic embryos to plantlets was stimulated by partial desic
dcterms:title
Vliv diferencované úspěšnosti somatické embryogeneze smrku ztepilého Differential success of somatic embryogenesis in random gene pool of Norway spruce Differential success of somatic embryogenesis in random gene pool of Norway spruce
skos:prefLabel
Differential success of somatic embryogenesis in random gene pool of Norway spruce Differential success of somatic embryogenesis in random gene pool of Norway spruce Vliv diferencované úspěšnosti somatické embryogeneze smrku ztepilého
skos:notation
RIV/60460709:41320/07:17535!RIV08-MSM-41320___
n3:strany
74;87
n3:aktivita
n18:Z
n3:aktivity
Z(MSM 414100007)
n3:cisloPeriodika
2
n3:dodaniDat
n7:2008
n3:domaciTvurceVysledku
n16:3564835 n16:4933877
n3:druhVysledku
n10:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
417293
n3:idVysledku
RIV/60460709:41320/07:17535
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Norway spruce, somatic embryogenesis, Picea abies, plant grow regulators
n3:klicoveSlovo
n4:plant%20grow%20regulators n4:Picea%20abies n4:Norway%20spruce n4:somatic%20embryogenesis
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[AE87AC945B34]
n3:nazevZdroje
Journal of Forest Science
n3:obor
n15:GK
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n7:2007
n3:svazekPeriodika
53
n3:tvurceVysledku
Mauleová, Michaela Vítámvás, Jan
n3:zamer
n6:MSM%20414100007
s:issn
1212-4834
s:numberOfPages
14
n17:organizacniJednotka
41320