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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F09%3A31054%21RIV13-MSM-41210___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments during which oocytes were repeatedly exposed to 2mM SNAP for a short time (10, 20 or 30 minutes) were more efficient with regard to the activation rate, even when the total exposure time did not exceed 4 hours. Parthenogenetic development was very limited after continuous treatment with 2mM SNAP. A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to the morula stage 0 vs. 18%; development to the blastocyst 0 vs. 7%; P < 0.05). However, this developmental rate was significantly lower (P < 0.05) than the development induced by conventional activation treatment with calcium ionophore (development to the morula stage, 23%; development to the blastocyst stage, 18%). When we combined pulsatile SNAP-treatment wit Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments during which oocytes were repeatedly exposed to 2mM SNAP for a short time (10, 20 or 30 minutes) were more efficient with regard to the activation rate, even when the total exposure time did not exceed 4 hours. Parthenogenetic development was very limited after continuous treatment with 2mM SNAP. A significantly higher proportion of developing parthenogenetic embryos was observed after the pulsatile treatment (development to the morula stage 0 vs. 18%; development to the blastocyst 0 vs. 7%; P < 0.05). However, this developmental rate was significantly lower (P < 0.05) than the development induced by conventional activation treatment with calcium ionophore (development to the morula stage, 23%; development to the blastocyst stage, 18%). When we combined pulsatile SNAP-treatment wit
dcterms:title
Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor
skos:prefLabel
Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor
skos:notation
RIV/60460709:41210/09:31054!RIV13-MSM-41210___
n3:aktivita
n16:P n16:Z
n3:aktivity
P(GA523/06/0295), Z(MSM6046070901), Z(MZE0002701401)
n3:cisloPeriodika
7
n3:dodaniDat
n11:2013
n3:domaciTvurceVysledku
n7:3477789 n7:6578969
n3:druhVysledku
n14:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
312388
n3:idVysledku
RIV/60460709:41210/09:31054
n3:jazykVysledku
n12:eng
n3:klicovaSlova
activation, nitric oxide, oocyte, pig
n3:klicoveSlovo
n4:oocyte n4:nitric%20oxide n4:activation n4:pig
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[DDFE35253C33]
n3:nazevZdroje
Czech Journal of Animal Science
n3:obor
n15:GG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n8:GA523%2F06%2F0295
n3:rokUplatneniVysledku
n11:2009
n3:svazekPeriodika
54
n3:tvurceVysledku
Petr, Jaroslav Kheilová, Kateřina Krejčová, Miroslava Krejčová, Tereza
n3:zamer
n10:MSM6046070901 n10:MZE0002701401
s:issn
1212-1819
s:numberOfPages
14
n18:organizacniJednotka
41210