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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F07%3A23442%21RIV08-MSM-41210___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5–1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplemented with 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium with 1 mg.l-1 2,4 Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5–1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplemented with 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium with 1 mg.l-1 2,4 Histological analysis of somatic embryogenesis and organogenesis derived from in vitro-grown leaves were assessed in Phlox paniculata L. Phlox cultivars ´Fuji´ and ´Starfire´ belong to important border perennials and are different in anthocyan occurrence. The leaf explants from sterile cultures were incubated on full-strenght MS salts (Murashige and Skoog, 1962) medium containing 0.5–1.0 mg.l-1 2,4-D (2,4-dichlorophenoxyacetic acid) with/without 0.2 mg.l-1 BA (benzyladenine). MS medium was supplemented with 30 or 90 g l-1 sucrose, B5 vitamins and 7.5 g l-1 agar Sigma. The intact leaf blades were cultured (abaxial side down) in the dark at temperature 22 +- 2 °C. The regeneration rate was expressed as the percentage of explant total number and the structures formed. The regeneration was highly depended on the genotype and concentration of 2,4-D and BA present in the culture medium. The significantly highest rate of regeneration (about 90 %) and callus induction were obtained on medium with 1 mg.l-1 2,4
dcterms:title
Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L. Iniciace a histologie somatické embryogeneze a organogeneze u Phlox paniculata L. Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L.
skos:prefLabel
Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L. Iniciace a histologie somatické embryogeneze a organogeneze u Phlox paniculata L. Initiation and histology of somatic embryogenesis and organogenesis in Phlox paniculata L.
skos:notation
RIV/60460709:41210/07:23442!RIV08-MSM-41210___
n4:strany
70;70
n4:aktivita
n8:Z
n4:aktivity
Z(MSM6046070901)
n4:dodaniDat
n11:2008
n4:domaciTvurceVysledku
n21:4498941
n4:druhVysledku
n20:D
n4:duvernostUdaju
n13:S
n4:entitaPredkladatele
n19:predkladatel
n4:idSjednocenehoVysledku
426689
n4:idVysledku
RIV/60460709:41210/07:23442
n4:jazykVysledku
n18:eng
n4:klicovaSlova
phlox paniculata, histology, somatic embryo, organogenesis
n4:klicoveSlovo
n6:phlox%20paniculata n6:histology n6:organogenesis n6:somatic%20embryo
n4:kontrolniKodProRIV
[BCCE5F760427]
n4:mistoKonaniAkce
Sofia, Bulgaria
n4:mistoVydani
Sofia, Bulgaria
n4:nazevEdiceCisloSvazku
Neuvedeno, 0
n4:nazevZdroje
Propagation of Ornamental Plants. Proc. of the 5th Intern. Conference “Propagation of Ornamental Plants
n4:obor
n12:ED
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
1
n4:rokUplatneniVysledku
n11:2007
n4:tvurceVysledku
Matiska, Pavel
n4:typAkce
n9:WRD
n4:zahajeniAkce
2007-09-05+02:00
n4:zamer
n14:MSM6046070901
s:numberOfPages
1
n10:hasPublisher
SEJANI Publisher
n17:isbn
978-954-90425-4-2
n7:organizacniJednotka
41210