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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F06%3A18178%21RIV07-MSM-41210___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
Apricot latent virus (ALV) is one of viruses infecting apricots mainly in Mediterranean countries. It is included in certification schemes of EPPO. That is why we adapted a PCR method for its detection. Positive control was obtained from Dr. Myrta from Italy. Buds from these sprouts were budded onto GF 305 rootstock in a greenhouse. Under these conditions these plants display no symptoms but they contain the virus. From three methods of RNA extraction from leaves that using phenol was the best. Primers were designed according to previous work of Nemchinov and Hadidi (1998). Predicted product lenght is 206 bp. Transcription is done using AMV RT at 42 °C. Conditions for PCR were optimized (72 °C for 5 min, 35 cycles of 94 °C, 50 °C and 72 °C for denaturation, annealing and DNA synthesis, respectively, and final 72 °C for 4 min) and this method was used for testing of leaf samples from apricot trees from commercial orchards in the Czech Republic during autumn 2005 and spring 2006. Until now no infected Apricot latent virus (ALV) is one of viruses infecting apricots mainly in Mediterranean countries. It is included in certification schemes of EPPO. That is why we adapted a PCR method for its detection. Positive control was obtained from Dr. Myrta from Italy. Buds from these sprouts were budded onto GF 305 rootstock in a greenhouse. Under these conditions these plants display no symptoms but they contain the virus. From three methods of RNA extraction from leaves that using phenol was the best. Primers were designed according to previous work of Nemchinov and Hadidi (1998). Predicted product lenght is 206 bp. Transcription is done using AMV RT at 42 °C. Conditions for PCR were optimized (72 °C for 5 min, 35 cycles of 94 °C, 50 °C and 72 °C for denaturation, annealing and DNA synthesis, respectively, and final 72 °C for 4 min) and this method was used for testing of leaf samples from apricot trees from commercial orchards in the Czech Republic during autumn 2005 and spring 2006. Until now no infected Apricot latent virus (ALV) is one of viruses infecting apricots mainly in Mediterranean countries. It is included in certification schemes of EPPO. That is why we adapted a PCR method for its detection. Positive control was obtained from Dr. Myrta from Italy. Buds from these sprouts were budded onto GF 305 rootstock in a greenhouse. Under these conditions these plants display no symptoms but they contain the virus. From three methods of RNA extraction from leaves that using phenol was the best. Primers were designed according to previous work of Nemchinov and Hadidi (1998). Predicted product lenght is 206 bp. Transcription is done using AMV RT at 42 °C. Conditions for PCR were optimized (72 °C for 5 min, 35 cycles of 94 °C, 50 °C and 72 °C for denaturation, annealing and DNA synthesis, respectively, and final 72 °C for 4 min) and this method was used for testing of leaf samples from apricot trees from commercial orchards in the Czech Republic during autumn 2005 and spring 2006. Until now no infected
dcterms:title
Development of RT- PCR for detection of Apricot latent virus and its survey in the Czech Republic Vývoj RT-PCR pro detekci latentního viru meruňky a jeho monitoring v ČR Development of RT- PCR for detection of Apricot latent virus and its survey in the Czech Republic
skos:prefLabel
Vývoj RT-PCR pro detekci latentního viru meruňky a jeho monitoring v ČR Development of RT- PCR for detection of Apricot latent virus and its survey in the Czech Republic Development of RT- PCR for detection of Apricot latent virus and its survey in the Czech Republic
skos:notation
RIV/60460709:41210/06:18178!RIV07-MSM-41210___
n3:strany
39;44
n3:aktivita
n17:Z
n3:aktivity
Z(MSM6046070901)
n3:dodaniDat
n6:2007
n3:domaciTvurceVysledku
n18:9926798 n18:2841495 n18:6059716
n3:druhVysledku
n7:D
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
471317
n3:idVysledku
RIV/60460709:41210/06:18178
n3:jazykVysledku
n4:eng
n3:klicovaSlova
ALV, RT-PCR, survey
n3:klicoveSlovo
n9:RT-PCR n9:ALV n9:survey
n3:kontrolniKodProRIV
[B4C38D95632F]
n3:mistoKonaniAkce
Praha
n3:mistoVydani
Praha
n3:nazevEdiceCisloSvazku
Neuvedeno, 0
n3:nazevZdroje
Proceedings of the 17th Czech and Slovak Plant Protection Conference, Prague, 12-14th September 2006,
n3:obor
n20:GF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n6:2006
n3:tvurceVysledku
Grimová, Lenka Zouhar, Miloslav Ryšánek, Pavel
n3:typAkce
n5:WRD
n3:zahajeniAkce
2006-09-12+02:00
n3:zamer
n16:MSM6046070901
s:numberOfPages
6
n15:hasPublisher
Česká zemědělská univerzita v Praze
n8:isbn
80-213-1516-4
n10:organizacniJednotka
41210