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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F05%3A11703%21RIV06-MSM-41210___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called “ageing”, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time. There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called “ageing”, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time. There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called “ageing”, which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured pig oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of EGF or IGF-1 had no significant effect on the ageing of pig oocytes. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.
dcterms:title
Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) Zlepšení vývojové kompetence stárnoucích oocytů po ošetření IGF1 a EGF
skos:prefLabel
Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) Improvement of developmental competence of aged porcine oocytes by means of the synergisti effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF) Zlepšení vývojové kompetence stárnoucích oocytů po ošetření IGF1 a EGF
skos:notation
RIV/60460709:41210/05:11703!RIV06-MSM-41210___
n3:strany
300;310
n3:aktivita
n10:Z
n3:aktivity
Z(MSM6046070901)
n3:cisloPeriodika
7
n3:dodaniDat
n12:2006
n3:domaciTvurceVysledku
n8:3761207 n8:8783004 n8:7540949 n8:1500015 n8:4182944
n3:druhVysledku
n18:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
524413
n3:idVysledku
RIV/60460709:41210/05:11703
n3:jazykVysledku
n16:eng
n3:klicovaSlova
epidermal growth factor, insulin-like growth factor 1, oocyte, ageing, pig
n3:klicoveSlovo
n7:epidermal%20growth%20factor n7:pig n7:insulin-like%20growth%20factor%201 n7:oocyte n7:ageing
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[3A588C8604E9]
n3:nazevZdroje
Czech Journal of Animal Science
n3:obor
n9:GG
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n12:2005
n3:svazekPeriodika
50
n3:tvurceVysledku
Rajmon, Radko Petrová, I. Sedmíková, Markéta Rozinek, Jiří Jílek, František Kuthanová, Zuzana
n3:zamer
n4:MSM6046070901
s:issn
1212-1819
s:numberOfPages
11
n5:organizacniJednotka
41210