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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F05%3A%23%23%2312265%21RIV06-MZE-41210___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detected in flower High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detected in flower High incidence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd) infections of peach trees was recently found in the Czech Republic. In the frame of prepared certification system for fruit trees planting material a PCR method for these purposes had to be developed. Several primer pairs were designed according to the published sequences of both viroids. Various plant material (leaves, flowers, pollen, bark) was tested during the year and also several methods of RNA extraction were tested.PCR with different primer pairs gives reliable results. More important is the time of testing, material, and RNA extraction method. The best period for detection of both viroids is late spring and early summer taking leaves as testing material and usingany of RNA extraction methods. For older leaves in late summer using of Qiagen kit for RNA extraction can be recommended. Detection of both viroids in bark is possible even during winter but it is not very reliable. Only HSVd can be detected in flower
dcterms:title
Vývoj metody RT-PCR pro detekci viroidu latentní mozaiky broskvoně a viroidu zakrslosti chmele pro účely certifikace sadbového materiálu Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material. Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material.
skos:prefLabel
Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material. Vývoj metody RT-PCR pro detekci viroidu latentní mozaiky broskvoně a viroidu zakrslosti chmele pro účely certifikace sadbového materiálu Development of a PCR method of Peach latent mosaic viroid and Hop stunt viroid detection for certification of planting material.
skos:notation
RIV/60460709:41210/05:###12265!RIV06-MZE-41210___
n3:strany
391;395
n3:aktivita
n11:P
n3:aktivity
P(QC1359)
n3:cisloPeriodika
657
n3:dodaniDat
n16:2006
n3:domaciTvurceVysledku
n9:6059716 Hassan, Mohamed Ahmed Ali n9:2841495
n3:druhVysledku
n8:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
517737
n3:idVysledku
RIV/60460709:41210/05:###12265
n3:jazykVysledku
n12:eng
n3:klicovaSlova
PLMVd, HSVd, RNA extraction, RT-PCR, peach, certification
n3:klicoveSlovo
n4:RT-PCR n4:PLMVd n4:HSVd n4:peach n4:certification n4:RNA%20extraction
n3:kodStatuVydavatele
BE - Belgické království
n3:kontrolniKodProRIV
[68F07F301468]
n3:nazevZdroje
Acta Horticulturae
n3:obor
n14:GF
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n15:QC1359
n3:rokUplatneniVysledku
n16:2005
n3:svazekPeriodika
5
n3:tvurceVysledku
Zouhar, Miloslav Ryšánek, Pavel Hassan, Mohamed Ahmed Ali
s:issn
0567-7572
s:numberOfPages
5
n13:organizacniJednotka
41210