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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F04%3A7932%21RIV%2F2005%2FMSM%2F412105%2FN
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as using PCR, bu Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as using PCR, bu Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg/l). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46°C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers. The bifidobacterial counts in products tested varied from 2.37 to 7.17 log CFU/ml. High selectivity was seen for modified TPY agar from which all isolates were identified as bifidobacteria. While most strains were identified as B. animalis using Bacter program, the same isolates had positive reaction with B. lactis-specific primers. Strain isolated from Rajo yogurt was identified as B. longum by Bacter as well as using PCR, bu
dcterms:title
Počty, isolace a identifikace bifidobakterií izolovaných z mléčných výrobků. Enumeration, isolation, and identification of bifidobacteria from dairy products. Enumeration, isolation, and identification of bifidobacteria from dairy products.
skos:prefLabel
Enumeration, isolation, and identification of bifidobacteria from dairy products. Enumeration, isolation, and identification of bifidobacteria from dairy products. Počty, isolace a identifikace bifidobakterií izolovaných z mléčných výrobků.
skos:notation
RIV/60460709:41210/04:7932!RIV/2005/MSM/412105/N
n3:strany
31;36
n3:aktivita
n12:Z
n3:aktivity
Z(MSM 412100003)
n3:cisloPeriodika
84
n3:dodaniDat
n9:2005
n3:domaciTvurceVysledku
n6:7344929 n6:4844092 n6:8865205
n3:druhVysledku
n15:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
562938
n3:idVysledku
RIV/60460709:41210/04:7932
n3:jazykVysledku
n4:eng
n3:klicovaSlova
milk products, dairy products, microbiology, bifidobacteria, enumeration, identification, isolation
n3:klicoveSlovo
n5:identification n5:milk%20products n5:enumeration n5:bifidobacteria n5:isolation n5:microbiology n5:dairy%20products
n3:kodStatuVydavatele
SI - Slovinská republika
n3:kontrolniKodProRIV
[B8985B8AB291]
n3:nazevZdroje
Acta Agriculturae Slovenica
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n9:2004
n3:svazekPeriodika
1
n3:tvurceVysledku
Vlková, Eva Rada, Vojtěch Trojanová, Iva
n3:zamer
n10:MSM%20412100003
s:issn
1581-9175
s:numberOfPages
6
n16:organizacniJednotka
41210