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Statements

Subject Item
n2:RIV%2F60460709%3A41210%2F04%3A7852%21RIV%2F2005%2FGA0%2F412105%2FN
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control, and four genetically modified lines of the same variety, were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene coding a yeast metallothionein), GUS (reporter gene for ß-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for ß-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, thus enabling to follow the heavy metal tolerance and uptake changes as function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with addition of cadmium, zinc and nickel. The results showed high Cd accumulation ability of HisCUP line. Determination of Zn content in above ground biomass did not confirm higher uptake by transgenic plants. The Ni content was significantly increased in above ground biomass of HisGUS construct. GUS construct introduced a Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control, and four genetically modified lines of the same variety, were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene coding a yeast metallothionein), GUS (reporter gene for ß-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for ß-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, thus enabling to follow the heavy metal tolerance and uptake changes as function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with addition of cadmium, zinc and nickel. The results showed high Cd accumulation ability of HisCUP line. Determination of Zn content in above ground biomass did not confirm higher uptake by transgenic plants. The Ni content was significantly increased in above ground biomass of HisGUS construct. GUS construct introduced a Tobacco, Nicotiana tabacum L., var. Wisconsin 38 as the control, and four genetically modified lines of the same variety, were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene coding a yeast metallothionein), GUS (reporter gene for ß-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for ß-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, thus enabling to follow the heavy metal tolerance and uptake changes as function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with addition of cadmium, zinc and nickel. The results showed high Cd accumulation ability of HisCUP line. Determination of Zn content in above ground biomass did not confirm higher uptake by transgenic plants. The Ni content was significantly increased in above ground biomass of HisGUS construct. GUS construct introduced a
dcterms:title
Příjem Cd, Zn a Ni geneticky modifikovanými liniemi tabáku The evaluation of Cd, Zn and Ni accumulation ability of transgenic tobacco bearing different transgenes Příjem Cd, Zn a Ni geneticky modifikovanými liniemi tabáku
skos:prefLabel
Příjem Cd, Zn a Ni geneticky modifikovanými liniemi tabáku Příjem Cd, Zn a Ni geneticky modifikovanými liniemi tabáku The evaluation of Cd, Zn and Ni accumulation ability of transgenic tobacco bearing different transgenes
skos:notation
RIV/60460709:41210/04:7852!RIV/2005/GA0/412105/N
n3:strany
513;517
n3:aktivita
n5:P
n3:aktivity
P(GA526/02/0293)
n3:cisloPeriodika
12
n3:dodaniDat
n6:2005
n3:domaciTvurceVysledku
n13:7052863 n13:2080400
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
581743
n3:idVysledku
RIV/60460709:41210/04:7852
n3:jazykVysledku
n8:eng
n3:klicovaSlova
transgenic tobacco, metallothionein, polyhistidine, ß-glucuronidase, cadmium, zinc, nickel, accumulation
n3:klicoveSlovo
n4:metallothionein n4:cadmium n4:accumulation n4:polyhistidine n4:transgenic%20tobacco n4:nickel n4:zinc n4:%C3%9F-glucuronidase
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[7AEF53B16BF4]
n3:nazevZdroje
Plant, Soil and Environment
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n12:GA526%2F02%2F0293
n3:rokUplatneniVysledku
n6:2004
n3:svazekPeriodika
50
n3:tvurceVysledku
Macková, Martina Száková, Jiřina Tlustoš, Pavel Pavlíková, Daniela Macek, Tomáš Surá, Martina
s:issn
1214-1178
s:numberOfPages
5
n9:organizacniJednotka
41210