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Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F14%3A43875204%21RIV15-MO0-G44_____
rdf:type
skos:Concept n13:Vysledek
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201400198/abstract;jsessionid=E6D0AB6647D589B559F50338BD786CD5.f02t02
dcterms:description
The posttranscriptional regulatory protein Hfq was shown to be an important determinant of the stress resistance and full virulence in the dangerous human pathogen Francisella tularensis. Transcriptomics brought rather limited clues to the precise contribution of Hfq in virulence. To reveal the molecular basis of the attenuation caused by hfq inactivation, we employed iTRAQ in the present study and compared proteomes of the parent and isogenic Delta hfq strains. We show that Hfq modulates the level of 76 proteins. Most of them show decreased abundance in the Delta hfq mutant, thereby indicating that Hfq widely acts rather as a positive regulator of Francisella gene expression. Several key Francisella virulence factors including those encoded within the Francisella pathogenicity island were found among the downregulated proteins, which is in a good agreement with the attenuated phenotype of the Delta hfq strain. To further validate the iTRAQ exploratory findings, we subsequently performed targeted LC-SRM analysis of selected proteins. This accurate quantification method corroborated the trends found in the iTRAQ data. The posttranscriptional regulatory protein Hfq was shown to be an important determinant of the stress resistance and full virulence in the dangerous human pathogen Francisella tularensis. Transcriptomics brought rather limited clues to the precise contribution of Hfq in virulence. To reveal the molecular basis of the attenuation caused by hfq inactivation, we employed iTRAQ in the present study and compared proteomes of the parent and isogenic Delta hfq strains. We show that Hfq modulates the level of 76 proteins. Most of them show decreased abundance in the Delta hfq mutant, thereby indicating that Hfq widely acts rather as a positive regulator of Francisella gene expression. Several key Francisella virulence factors including those encoded within the Francisella pathogenicity island were found among the downregulated proteins, which is in a good agreement with the attenuated phenotype of the Delta hfq strain. To further validate the iTRAQ exploratory findings, we subsequently performed targeted LC-SRM analysis of selected proteins. This accurate quantification method corroborated the trends found in the iTRAQ data.
dcterms:title
Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype
skos:prefLabel
Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype Changes in proteome of the Delta hfq strain derived from Francisella tularensis LVS correspond with its attenuated phenotype
skos:notation
RIV/60162694:G44__/14:43875204!RIV15-MO0-G44_____
n3:aktivita
n15:I
n3:aktivity
I
n3:cisloPeriodika
21-22
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n10:4600622 n10:5863163 n10:1768336 n10:2231263 n10:9267336
n3:druhVysledku
n14:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
6813
n3:idVysledku
RIV/60162694:G44__/14:43875204
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Virulence; Microbiology; Mass spectrometry; Hfq; Francisella tularensis
n3:klicoveSlovo
n9:Francisella%20tularensis n9:Virulence n9:Microbiology n9:Hfq n9:Mass%20spectrometry
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[285FF8DE8E50]
n3:nazevZdroje
Proteomics
n3:obor
n4:EE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
14
n3:tvurceVysledku
Charbit, Alain Peterek, Miroslav Klimentová, Jana Lenčo, Juraj Dresler, Jiří Stulík, Jiří Link, Marek Tambor, Vojtěch
n3:wos
000344807600003
s:issn
1615-9853
s:numberOfPages
10
n8:doi
10.1002/pmic.201400198
n16:organizacniJednotka
G44