This HTML5 document contains 56 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n16http://localhost/temp/predkladatel/
n7http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n12http://linked.opendata.cz/ontology/domain/vavai/
n18http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n15http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F60162694%3AG44__%2F12%3A43874693%21RIV13-MO0-G44_____/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n11http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n5http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F12%3A43874693%21RIV13-MO0-G44_____
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://www.lfhk.cuni.cz/default.asp?nDepartmentID=1286&nLanguageID=2
dcterms:description
In this paper we describe the influence of NU7026, a specific inhibitor of DNA-dependent protein kinase, phosphoinositide 3-kinase, and ATM-kinase on molecular and cellular mechanisms triggered by ionising irradiation in human T-lymphocyte leukaemic MOLT-4 cells. We studied the effect of this inhibitor (10 1microM) combined with gamma-radiation (1 Gy) leading to DNA damage response and induction of apoptosis. We used methods for apoptosis assessment (cell viability count and flow-cytometric analysis) and cell cycle analysis (DNA content measurement) and we detected expression and post-translational modifications (Western blotting) of proteins involved in DNA repair signalling pathways. Pre-treatment with NU7026 resulted into decreased activation of checkpoint kinase-2 (Thr68), p53 (Ser15 and Ser392), and histone H2A.X (Ser139) 2 hours after irradiation. Subsequently, combination of radiation and inhibitor led to decreased amount of cells in G2-phase arrest and into increased apoptosis after 72 hours. Our results indicate that in leukaemic cells the pre-incubation with inhibitor NU7026 followed by low doses of ionising radiation results in radio-sensitising of MOLT-4 cells via diminished DNA repair and delayed but pronounced apoptosis. This novel approach might offer new strategies in combined treatment of leukaemia diseases. In this paper we describe the influence of NU7026, a specific inhibitor of DNA-dependent protein kinase, phosphoinositide 3-kinase, and ATM-kinase on molecular and cellular mechanisms triggered by ionising irradiation in human T-lymphocyte leukaemic MOLT-4 cells. We studied the effect of this inhibitor (10 1microM) combined with gamma-radiation (1 Gy) leading to DNA damage response and induction of apoptosis. We used methods for apoptosis assessment (cell viability count and flow-cytometric analysis) and cell cycle analysis (DNA content measurement) and we detected expression and post-translational modifications (Western blotting) of proteins involved in DNA repair signalling pathways. Pre-treatment with NU7026 resulted into decreased activation of checkpoint kinase-2 (Thr68), p53 (Ser15 and Ser392), and histone H2A.X (Ser139) 2 hours after irradiation. Subsequently, combination of radiation and inhibitor led to decreased amount of cells in G2-phase arrest and into increased apoptosis after 72 hours. Our results indicate that in leukaemic cells the pre-incubation with inhibitor NU7026 followed by low doses of ionising radiation results in radio-sensitising of MOLT-4 cells via diminished DNA repair and delayed but pronounced apoptosis. This novel approach might offer new strategies in combined treatment of leukaemia diseases.
dcterms:title
Radio-sensitization of human leukaemic molt-4 cells by DNA-dependent protein kinase inhibitor, NU7026 Radio-sensitization of human leukaemic molt-4 cells by DNA-dependent protein kinase inhibitor, NU7026
skos:prefLabel
Radio-sensitization of human leukaemic molt-4 cells by DNA-dependent protein kinase inhibitor, NU7026 Radio-sensitization of human leukaemic molt-4 cells by DNA-dependent protein kinase inhibitor, NU7026
skos:notation
RIV/60162694:G44__/12:43874693!RIV13-MO0-G44_____
n4:aktivita
n11:I n11:Z
n4:aktivity
I, Z(MO0FVZ0000501)
n4:cisloPeriodika
2
n4:dodaniDat
n5:2013
n4:domaciTvurceVysledku
n7:5654823 n7:4404394 n7:3518043 n7:8885028 n7:6501370 n7:6389627 n7:5470110
n4:druhVysledku
n9:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
163966
n4:idVysledku
RIV/60162694:G44__/12:43874693
n4:jazykVysledku
n10:eng
n4:klicovaSlova
cell cycle; apoptosis; leukaemia; P13-K; Ionizing radiation
n4:klicoveSlovo
n13:apoptosis n13:cell%20cycle n13:P13-K n13:leukaemia n13:Ionizing%20radiation
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[7FFB3E2C16D4]
n4:nazevZdroje
Acta Medica (Hradec Králové)
n4:obor
n6:FD
n4:pocetDomacichTvurcuVysledku
7
n4:pocetTvurcuVysledku
10
n4:rokUplatneniVysledku
n5:2012
n4:svazekPeriodika
55
n4:tvurceVysledku
Zárybnická, Lenka Sedlaříková, Radka Ďurišová, Kamila Šinkorová, Zuzana Vávrová, Jiřina Pejchal, Jaroslav Řezáčová, Martina Šalovská, Barbora Tichý, Aleš Novotná, Eva
n4:zamer
n18:MO0FVZ0000501
s:issn
1211-4286
s:numberOfPages
8
n16:organizacniJednotka
G44