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Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F11%3A00002561%21RIV12-MO0-G44_____
rdf:type
skos:Concept n13:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.3390/ijms12032077
dcterms:description
We have in vitro tested the ability of common, commercially available, cholinesterase reactivators (pralidoxime, obidoxime, methoxime, trimedoxime and HI-6) to reactivate human acetylcholinesterase (AChE), inhibited by five structurally different organophosphate pesticides and inhibitors (paraoxon, dichlorvos, DFP, leptophos-oxon and methamidophos). We also tested reactivation of human butyrylcholinesterase (BChE) with the aim of finding a potent oxime, suitable to serve as a %22pseudocatalytic%22 bioscavenger in combination with this enzyme. Such a combination could allow an increase of prophylactic and therapeutic efficacy of the administered enzyme. According to our results, the best broad-spectrum AChE reactivators were trimedoxime and obidoxime in the case of paraoxon, leptophos-oxon, and methamidophos-inhibited AChE. Methamidophos and leptophos-oxon were quite easily reactivatable by all tested reactivators. In the case of methamidophos-inhibited AChE, the lower oxime concentration (10(-5) M) had higher reactivation ability than the 10 (4) M concentration. Therefore, we evaluated the reactivation ability of obidoxime in a concentration range of 10(-3)-10(-7) M. The reactivation of methamidophos-inhibited AChE with different obidoxime concentrations resulted in a bell shaped curve with maximum reactivation at 10(-5) M. In the case of BChE, no reactivator exceeded 15% reactivation ability and therefore none of the oximes can be recommended as a candidate for %22pseudocatalytic%22 bioscavengers with BChE. We have in vitro tested the ability of common, commercially available, cholinesterase reactivators (pralidoxime, obidoxime, methoxime, trimedoxime and HI-6) to reactivate human acetylcholinesterase (AChE), inhibited by five structurally different organophosphate pesticides and inhibitors (paraoxon, dichlorvos, DFP, leptophos-oxon and methamidophos). We also tested reactivation of human butyrylcholinesterase (BChE) with the aim of finding a potent oxime, suitable to serve as a %22pseudocatalytic%22 bioscavenger in combination with this enzyme. Such a combination could allow an increase of prophylactic and therapeutic efficacy of the administered enzyme. According to our results, the best broad-spectrum AChE reactivators were trimedoxime and obidoxime in the case of paraoxon, leptophos-oxon, and methamidophos-inhibited AChE. Methamidophos and leptophos-oxon were quite easily reactivatable by all tested reactivators. In the case of methamidophos-inhibited AChE, the lower oxime concentration (10(-5) M) had higher reactivation ability than the 10 (4) M concentration. Therefore, we evaluated the reactivation ability of obidoxime in a concentration range of 10(-3)-10(-7) M. The reactivation of methamidophos-inhibited AChE with different obidoxime concentrations resulted in a bell shaped curve with maximum reactivation at 10(-5) M. In the case of BChE, no reactivator exceeded 15% reactivation ability and therefore none of the oximes can be recommended as a candidate for %22pseudocatalytic%22 bioscavengers with BChE.
dcterms:title
In vitro ability of currently available oximes to reactivate organophosphate pesticide-inhibited human acetylcholinesterase and butyrylcholinesterase In vitro ability of currently available oximes to reactivate organophosphate pesticide-inhibited human acetylcholinesterase and butyrylcholinesterase
skos:prefLabel
In vitro ability of currently available oximes to reactivate organophosphate pesticide-inhibited human acetylcholinesterase and butyrylcholinesterase In vitro ability of currently available oximes to reactivate organophosphate pesticide-inhibited human acetylcholinesterase and butyrylcholinesterase
skos:notation
RIV/60162694:G44__/11:00002561!RIV12-MO0-G44_____
n4:aktivita
n12:I n12:S n12:P n12:Z
n4:aktivity
I, P(OVUOFVZ200902), S, Z(MO0FVZ0000604)
n4:cisloPeriodika
3
n4:dodaniDat
n7:2012
n4:domaciTvurceVysledku
n14:4185137 n14:6469922 n14:6306888
n4:druhVysledku
n10:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n20:predkladatel
n4:idSjednocenehoVysledku
204026
n4:idVysledku
RIV/60162694:G44__/11:00002561
n4:jazykVysledku
n11:eng
n4:klicovaSlova
acetylcholinesterase; butyrylcholinesterase; in vitro; nerve agent; organophosphate; pesticide; reactivator; oxime; scavenger
n4:klicoveSlovo
n8:nerve%20agent n8:butyrylcholinesterase n8:acetylcholinesterase n8:organophosphate n8:reactivator n8:pesticide n8:oxime n8:in%20vitro n8:scavenger
n4:kodStatuVydavatele
CH - Švýcarská konfederace
n4:kontrolniKodProRIV
[82EB5091E922]
n4:nazevZdroje
International Journal of Molecular Sciences
n4:obor
n5:FP
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n6:OVUOFVZ200902
n4:rokUplatneniVysledku
n7:2011
n4:svazekPeriodika
12
n4:tvurceVysledku
Jun, Daniel Musilová, Lucie Kuča, Kamil Musílek, Kamil
n4:wos
000288784200043
n4:zamer
n16:MO0FVZ0000604
s:issn
1422-0067
s:numberOfPages
11
n19:organizacniJednotka
G44