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Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F11%3A00002471%21RIV12-MO0-G44_____
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.2478/v10136-009-0031-7
dcterms:description
In this study we used caffeine, a non-specific ATM inhibitor, and studied its effect on the activation of the proteins involved in cell cycle control and the induction of apoptosis in human T-lymphocyte leukaemic MOLT-4 cells (p53 wt) after irradiation. We evaluated the expression of the tumour-suppressor p53, the cell cycle regulator p21, and the anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1). After treatment with 2 mM caffeine, the cells were irradiated by 1 or 3 Gy. The ATM/p53 pathway was suppressed, which led to increased apoptosis accompanied by a p53-independent decrease in Mcl-1. It also caused down-regulation of p21, which possibly contributed to the shortened cell cycle arrest necessary for effective DNA repair and thus impeded radio-resistance. Caffeine promotes the cytotoxic effect of ionising radiation and provides a possible platform for the development of new anti-cancer therapeutics known as radio-sensitizers. In this study we used caffeine, a non-specific ATM inhibitor, and studied its effect on the activation of the proteins involved in cell cycle control and the induction of apoptosis in human T-lymphocyte leukaemic MOLT-4 cells (p53 wt) after irradiation. We evaluated the expression of the tumour-suppressor p53, the cell cycle regulator p21, and the anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1). After treatment with 2 mM caffeine, the cells were irradiated by 1 or 3 Gy. The ATM/p53 pathway was suppressed, which led to increased apoptosis accompanied by a p53-independent decrease in Mcl-1. It also caused down-regulation of p21, which possibly contributed to the shortened cell cycle arrest necessary for effective DNA repair and thus impeded radio-resistance. Caffeine promotes the cytotoxic effect of ionising radiation and provides a possible platform for the development of new anti-cancer therapeutics known as radio-sensitizers.
dcterms:title
Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells
skos:prefLabel
Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells Caffeine-suppressed ATM pathway leads to decreased p53 phosphorylation and increased programmed cell death in gamma-irradiated leukaemic molt-4 cells
skos:notation
RIV/60162694:G44__/11:00002471!RIV12-MO0-G44_____
n3:aktivita
n15:Z
n3:aktivity
Z(MO0FVZ0000501), Z(MSM0021620820)
n3:cisloPeriodika
1
n3:dodaniDat
n18:2012
n3:domaciTvurceVysledku
n13:5470110 n13:6389627 n13:6501370 n13:8885028 n13:4404394
n3:druhVysledku
n9:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
188999
n3:idVysledku
RIV/60162694:G44__/11:00002471
n3:jazykVysledku
n7:eng
n3:klicovaSlova
IR; ionising radiation; ATM; ataxia telangiectasia mutated kinase; ATR; ATM- and Rad3-related protein kinase; chk-2; checkpoint kinase-2
n3:klicoveSlovo
n8:ionising%20radiation n8:checkpoint%20kinase-2 n8:ataxia%20telangiectasia%20mutated%20kinase n8:chk-2 n8:ATM n8:IR n8:ATM-%20and%20Rad3-related%20protein%20kinase n8:ATR
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[81D41F987CCA]
n3:nazevZdroje
Journal of Applied Biomedicine - print
n3:obor
n19:BO
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:rokUplatneniVysledku
n18:2011
n3:svazekPeriodika
9
n3:tvurceVysledku
Pejchal, Jaroslav Vávrová, Jiřina Zárybnická, Lenka Tichý, Aleš Muthná, Darina Řezáčová, Martina Šinkorová, Zuzana
n3:wos
000284780800004
n3:zamer
n4:MO0FVZ0000501 n4:MSM0021620820
s:issn
1214-021X
s:numberOfPages
8
n14:organizacniJednotka
G44