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Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F10%3A00002374%21RIV11-MO0-G44_____
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Bioscavengers are considered as promising antidotes against organophosphate poisoning. We focused on a bacterial phosphotriesterase (PTE) expressed in Escherichia coli. The main disadvantage of this non-human catalytic bioscavenger is its relatively short half-life in the organism and strong immunogenicity after repeated administration. Therefore, we prepared different methoxy polyethylene glycol (MPEG) conjugated recombinant PTE as a potential catalytic bioscavenger with the aim to improve its biological properties. Enzyme was modified with two linear monofunctional MPEG derivatives with reactive aldehyde group of molecular weight 2 kDa and 5 kDa. We optimized reaction conditions (reagent ratios, temperature and duration of modification reaction) and we prepared homogeneous population of fully modified recombinant PTE with molecular weight around 52 kDa and 76 kDa, respectively. Modified PTE was characterized using SDS-PAGE and MALDI-TOF and by determining K-m and V-max. Bioscavengers are considered as promising antidotes against organophosphate poisoning. We focused on a bacterial phosphotriesterase (PTE) expressed in Escherichia coli. The main disadvantage of this non-human catalytic bioscavenger is its relatively short half-life in the organism and strong immunogenicity after repeated administration. Therefore, we prepared different methoxy polyethylene glycol (MPEG) conjugated recombinant PTE as a potential catalytic bioscavenger with the aim to improve its biological properties. Enzyme was modified with two linear monofunctional MPEG derivatives with reactive aldehyde group of molecular weight 2 kDa and 5 kDa. We optimized reaction conditions (reagent ratios, temperature and duration of modification reaction) and we prepared homogeneous population of fully modified recombinant PTE with molecular weight around 52 kDa and 76 kDa, respectively. Modified PTE was characterized using SDS-PAGE and MALDI-TOF and by determining K-m and V-max.
dcterms:title
Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning
skos:prefLabel
Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning Preparation and characterization of methoxy polyethylene glycol-conjugated phosphotriesterase as a potential catalytic bioscavenger against organophosphate poisoning
skos:notation
RIV/60162694:G44__/10:00002374!RIV11-MO0-G44_____
n3:aktivita
n15:V n15:Z n15:P
n3:aktivity
P(IAA603020901), V, Z(MO0FVZ0000604)
n3:cisloPeriodika
1-3
n3:dodaniDat
n5:2011
n3:domaciTvurceVysledku
n12:2231263
n3:druhVysledku
n19:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
281334
n3:idVysledku
RIV/60162694:G44__/10:00002374
n3:jazykVysledku
n6:eng
n3:klicovaSlova
phosphotriesterase; acetylcholinesterase; butyrylcholinesterase; organophosphate; scavenger; bioscavenger
n3:klicoveSlovo
n8:butyrylcholinesterase n8:acetylcholinesterase n8:organophosphate n8:scavenger n8:phosphotriesterase n8:bioscavenger
n3:kodStatuVydavatele
IE - Irsko
n3:kontrolniKodProRIV
[F390C739A8C2]
n3:nazevZdroje
Chemico-Biological Interactions
n3:obor
n16:FP
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
8
n3:projekt
n13:IAA603020901
n3:rokUplatneniVysledku
n5:2010
n3:svazekPeriodika
187
n3:tvurceVysledku
Link, Marek Masson, Patrick Jun, Daniel Loiodice, Melanie Nachon, Florian Rochu, Daniel Musilová, Lucie Renault, Frederique
n3:wos
000281108600069
n3:zamer
n17:MO0FVZ0000604
s:issn
0009-2797
s:numberOfPages
4
n14:organizacniJednotka
G44