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Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F10%3A00002361%21RIV11-MO0-G44_____
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Peptide samples derived from enzymatic in-gel digestion of proteins resolved by gel electrophoresis often contain high amount of salts originating from reaction and separation buffers. Different methods are used for desalting prior to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), e.g. reversed-phase pipette tip purification, on-target washing, adding co-matrices, etc. As a suitable matrix for MALDI MS of peptides, alpha-cyano-4-hydroxycinnamic acid (CHCA) is frequently used. Crystalline CHCA shows the ability to bind peptides on its surface and because it is almost insoluble in acidic water solutions, the on-target washing of peptide samples can significantly improve MALDI MS signals. Although the common on-target washing represents a simple, cheap and fast procedure, only a small portion of the available peptide solution is efficiently used for the subsequent MS analysis. The present approach is a combination of the on-target washing principle carried out in a narrow-end Peptide samples derived from enzymatic in-gel digestion of proteins resolved by gel electrophoresis often contain high amount of salts originating from reaction and separation buffers. Different methods are used for desalting prior to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), e.g. reversed-phase pipette tip purification, on-target washing, adding co-matrices, etc. As a suitable matrix for MALDI MS of peptides, alpha-cyano-4-hydroxycinnamic acid (CHCA) is frequently used. Crystalline CHCA shows the ability to bind peptides on its surface and because it is almost insoluble in acidic water solutions, the on-target washing of peptide samples can significantly improve MALDI MS signals. Although the common on-target washing represents a simple, cheap and fast procedure, only a small portion of the available peptide solution is efficiently used for the subsequent MS analysis. The present approach is a combination of the on-target washing principle carried out in a narrow-end
dcterms:title
A convenient purification and preconcentration of peptides with alpha-cyano-4-hydroxycinnamic acid matrix crystals in a pipette tip for matrix-assisted laser desorption/ionization mass spectrometry A convenient purification and preconcentration of peptides with alpha-cyano-4-hydroxycinnamic acid matrix crystals in a pipette tip for matrix-assisted laser desorption/ionization mass spectrometry
skos:prefLabel
A convenient purification and preconcentration of peptides with alpha-cyano-4-hydroxycinnamic acid matrix crystals in a pipette tip for matrix-assisted laser desorption/ionization mass spectrometry A convenient purification and preconcentration of peptides with alpha-cyano-4-hydroxycinnamic acid matrix crystals in a pipette tip for matrix-assisted laser desorption/ionization mass spectrometry
skos:notation
RIV/60162694:G44__/10:00002361!RIV11-MO0-G44_____
n4:aktivita
n16:Z
n4:aktivity
Z(MO0FVZ0000501)
n4:cisloPeriodika
1
n4:dodaniDat
n14:2011
n4:domaciTvurceVysledku
n12:3062600
n4:druhVysledku
n8:J
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
244568
n4:idVysledku
RIV/60162694:G44__/10:00002361
n4:jazykVysledku
n6:eng
n4:klicovaSlova
alpha-cyano-4-hydroxycinnamic acid; desalting; MALDI matrix; peptide; pipette tip
n4:klicoveSlovo
n7:pipette%20tip n7:alpha-cyano-4-hydroxycinnamic%20acid n7:peptide n7:MALDI%20matrix n7:desalting
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[55343C3E09DC]
n4:nazevZdroje
Journal of Mass Spectrometry
n4:obor
n18:KA
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
4
n4:rokUplatneniVysledku
n14:2010
n4:svazekPeriodika
45
n4:tvurceVysledku
Řehulka, Pavel Šebela, Marek Chalupová, Jana Řehulková, Helena
n4:wos
000279253300017
n4:zamer
n10:MO0FVZ0000501
s:issn
1076-5174
s:numberOfPages
8
n13:organizacniJednotka
G44