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Statements

Subject Item
n2:RIV%2F60162694%3AG44__%2F08%3A00002011%21RIV09-MO0-G44_____
rdf:type
skos:Concept n19:Vysledek
dcterms:description
Wheat belongs to six major food allergens inducing IgE-mediated hypersensitivity reaction manifesting as cutaneous, gastrointestinal, and respiratory symptoms. Although cereals are a staple food item in most diets, only a few wheat proteins causing hypersensitivity have been identified. To characterize wheat allergens, salt-soluble wheat extracts were separated by 1-DE and 2-DE and IgE-binding proteins were detected by immunoblotting using sera of patients with allergy to ingested wheat. Proteins, frequently recognized by IgE on 2-DE were analyzed by MALDI-TOF and QTOF and their spectrum was completed by 1-DE and LCQ(DECA) nLC-MS/MS IT technique. Using all three techniques we identified 19 potential wheat allergens such as alpha-amylase inhibitors, beta-amylase, profilin, serpin, beta-D-glucan exohydrolase, and 27K protein. Employing newly developed ELISA, levels of IgE Abs against Sulamit wheat extract and alpha-amylase inhibitors type 1 and 3 were quantified and shown to be significantly elevated in Wheat belongs to six major food allergens inducing IgE-mediated hypersensitivity reaction manifesting as cutaneous, gastrointestinal, and respiratory symptoms. Although cereals are a staple food item in most diets, only a few wheat proteins causing hypersensitivity have been identified. To characterize wheat allergens, salt-soluble wheat extracts were separated by 1-DE and 2-DE and IgE-binding proteins were detected by immunoblotting using sera of patients with allergy to ingested wheat. Proteins, frequently recognized by IgE on 2-DE were analyzed by MALDI-TOF and QTOF and their spectrum was completed by 1-DE and LCQ(DECA) nLC-MS/MS IT technique. Using all three techniques we identified 19 potential wheat allergens such as alpha-amylase inhibitors, beta-amylase, profilin, serpin, beta-D-glucan exohydrolase, and 27K protein. Employing newly developed ELISA, levels of IgE Abs against Sulamit wheat extract and alpha-amylase inhibitors type 1 and 3 were quantified and shown to be significantly elevated in Pšenice patří mezi 6 hlavních potravinových alergenů vyvolávající hypersenzitivní reakci zprostředkovanou IgE protilátkami. V této studii bylo identifikováno 19 pšeničních antigenů, které byly rozpoznávány IgE protilátkami od séry pacientů s alergií na pšeničné antigeny.
dcterms:title
Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients Proteomická analýza pšeničných proteinů rozpoznávaná IgE protilátkami alergických pacientů Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients
skos:prefLabel
Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients Proteomic analysis of wheat proteins recognized by IgE antibodies of allergic patients Proteomická analýza pšeničných proteinů rozpoznávaná IgE protilátkami alergických pacientů
skos:notation
RIV/60162694:G44__/08:00002011!RIV09-MO0-G44_____
n4:aktivita
n11:P n11:Z
n4:aktivity
P(1QS500200572), P(2B06155), P(GA310/05/2245), P(GA310/07/0414), P(GD310/03/H147), P(IAA500200801), P(KJB5020407), P(LC07017), Z(AV0Z50200510), Z(MO0FVZ0000501)
n4:cisloPeriodika
8
n4:dodaniDat
n12:2009
n4:domaciTvurceVysledku
n14:4407989 n14:3764915 n14:5863163
n4:druhVysledku
n6:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
390642
n4:idVysledku
RIV/60162694:G44__/08:00002011
n4:jazykVysledku
n7:eng
n4:klicovaSlova
Basophil activation; IgE antibodies; wheat allergens
n4:klicoveSlovo
n8:Basophil%20activation n8:IgE%20antibodies n8:wheat%20allergens
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[D1347675F2F2]
n4:nazevZdroje
Proteomics
n4:obor
n15:EC
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
10
n4:projekt
n5:IAA500200801 n5:LC07017 n5:KJB5020407 n5:2B06155 n5:1QS500200572 n5:GA310%2F05%2F2245 n5:GA310%2F07%2F0414 n5:GD310%2F03%2FH147
n4:rokUplatneniVysledku
n12:2008
n4:svazekPeriodika
8
n4:tvurceVysledku
Hernychová, Lenka Šotkovský, Petr Novák, Petr Hubálek, Martin Šetinová, Iva Kitanovicová, Alena Fuchs, Milan Stulík, Jiří Havranová, Milada Tučková, Ludmila
n4:wos
000255314300013
n4:zamer
n9:MO0FVZ0000501 n9:AV0Z50200510
s:issn
1615-9853
s:numberOfPages
15
n18:organizacniJednotka
G44