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Statements

Subject Item
n2:RIV%2F60162694%3AG38__%2F01%3A00000499%21RIV%2F2005%2FMO0%2FG38005%2FN
rdf:type
skos:Concept n19:Vysledek
dcterms:description
We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight proteins were unambigu We have started the construction of a two dimensional database of the proteome of Francisella tularensis, a bacterium that is responsible for the highly pathogenic disease tularemia. The genome of this intracellular pathogen is not completely sequenced,yet and, currently, information only about 54 proteins is available from NCBI database. We have analyzed the Francisella tularensis live vaccine strain by two-dimensional gel electrophoresis with immobilized pH 3-10 gradient in the first dimension and 9-16 % gradient or tricine SDS-PAGE in the second dimension. In both cases about 2000 spots were detected. Furthermore, we compared the protein pattern of the non-virulent Francisella tularensis live vaccine strain with protein profiles of two wild type clinical isolates and more than 50 differentially expressed proteins were counted. The separated proteins are going to be identified by peptide mass fingerprinting, however, due to the lack of complete genome sequence data only eight proteins were unambigu Konstrukce 2-DE proteinové databáze Francisella tularensis
dcterms:title
Konstrukce 2-DE proteinové databáze Francisella tularensis Construction of a Francisella tularensis two-dimensional electrophoresis protein database Construction of a Francisella tularensis two-dimensional electrophoresis protein database
skos:prefLabel
Construction of a Francisella tularensis two-dimensional electrophoresis protein database Konstrukce 2-DE proteinové databáze Francisella tularensis Construction of a Francisella tularensis two-dimensional electrophoresis protein database
skos:notation
RIV/60162694:G38__/01:00000499!RIV/2005/MO0/G38005/N
n3:strany
508-515
n3:aktivita
n13:P n13:Z
n3:aktivity
P(LN00A033), P(MO66021299105), P(NI4700), Z(AV0Z5020903)
n3:cisloPeriodika
4
n3:dodaniDat
n7:2005
n3:domaciTvurceVysledku
n10:5863163 n10:6007600 n10:3764915 n10:3410722 n10:3813967
n3:druhVysledku
n6:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
676304
n3:idVysledku
RIV/60162694:G38__/01:00000499
n3:jazykVysledku
n9:eng
n3:klicovaSlova
Francisella tularensis;two-dimensional electrophoresis;protein database
n3:klicoveSlovo
n11:two-dimensional%20electrophoresis n11:Francisella%20tularensis n11:protein%20database
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[225857A9131C]
n3:nazevZdroje
Proteomics
n3:obor
n18:EC
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:projekt
n5:MO66021299105 n5:LN00A033 n5:NI4700
n3:rokUplatneniVysledku
n7:2001
n3:svazekPeriodika
1
n3:tvurceVysledku
Macela, Aleš Malina, Michal Hernychová, Lenka Johansson, Anders Kroča, Michal Halada, Petr Stulík, Jiří
n3:zamer
n4:AV0Z5020903
s:issn
1615-9853
s:numberOfPages
8
n17:organizacniJednotka
G38