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Statements

Subject Item
n2:RIV%2F60109807%3A_____%2F12%3A%230000202%21RIV13-MSM-60109807
rdf:type
n5:Vysledek skos:Concept
dcterms:description
The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET- 45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigens were used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus. The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET- 45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigens were used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus.
dcterms:title
Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein
skos:prefLabel
Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein
skos:notation
RIV/60109807:_____/12:#0000202!RIV13-MSM-60109807
n5:predkladatel
n9:ico%3A60109807
n3:aktivita
n13:Z n13:P
n3:aktivity
P(1M06030), Z(AV0Z50380511)
n3:cisloPeriodika
2012.01886
n3:dodaniDat
n8:2013
n3:domaciTvurceVysledku
n6:1745425
n3:druhVysledku
n15:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
162482
n3:idVysledku
RIV/60109807:_____/12:#0000202
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Potato virus M, recombinant protein, coat protein, triple gene block, polyclonal antibodies
n3:klicoveSlovo
n4:triple%20gene%20block n4:polyclonal%20antibodies n4:recombinant%20protein n4:coat%20protein n4:Potato%20virus%20M
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[65455F69913B]
n3:nazevZdroje
J Phytopathol
n3:obor
n11:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n16:1M06030
n3:rokUplatneniVysledku
n8:2012
n3:svazekPeriodika
160
n3:tvurceVysledku
Čeřovská, Noemi Hoffmeisterová, Hana Dědič, Petr Plchová, Helena Moravec, Tomáš
n3:zamer
n14:AV0Z50380511
s:issn
1439-0434
s:numberOfPages
3