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Statements

Subject Item
n2:RIV%2F60109807%3A_____%2F07%3A%230000073%21RIV08-MSM-60109807
rdf:type
n4:Vysledek skos:Concept
dcterms:description
Byla vybrána optimální metoda aklimatizace somatických hybridů rodu Solanum z in vitro do in vivo podmínek. Many valuable resistance genes are carried by wild species of Solanum genus that are often non-crossable with cultivated potato. Somatic hybridization is an approach overcoming this problem. The somatic hybrids were developed through protoplast fusion by an electric field (Navrátilová et al. 2006), they were evaluated by flow-cytometry, RAPD technique and their in vitro morphology. Optimal method for the most effective transfer into in vivo conditions was investigated. A set of somatic hybrids of two combinations was planted into seedling pots (3x3 cm) containing sterilized soil and cultured in a laboratory with artificial illumination (4000 Lux) of 16 h light/8 h dark photoperiod at 21 °C. The second variant involved direct planting into the pots (8x8 cm). Plants planted in this way were partially protected by non-woven fleece and exposed to weather conditions. Rate of survival and growth increment was investigated in both variants. Many valuable resistance genes are carried by wild species of Solanum genus that are often non-crossable with cultivated potato. Somatic hybridization is an approach overcoming this problem. The somatic hybrids were developed through protoplast fusion by an electric field (Navrátilová et al. 2006), they were evaluated by flow-cytometry, RAPD technique and their in vitro morphology. Optimal method for the most effective transfer into in vivo conditions was investigated. A set of somatic hybrids of two combinations was planted into seedling pots (3x3 cm) containing sterilized soil and cultured in a laboratory with artificial illumination (4000 Lux) of 16 h light/8 h dark photoperiod at 21 °C. The second variant involved direct planting into the pots (8x8 cm). Plants planted in this way were partially protected by non-woven fleece and exposed to weather conditions. Rate of survival and growth increment was investigated in both variants.
dcterms:title
Acclimatization of the somatic hybrids in the Solanum genus Acclimatization of the somatic hybrids in the Solanum genus Aklimatizace somatických hybridů rodu Solanum
skos:prefLabel
Acclimatization of the somatic hybrids in the Solanum genus Acclimatization of the somatic hybrids in the Solanum genus Aklimatizace somatických hybridů rodu Solanum
skos:notation
RIV/60109807:_____/07:#0000073!RIV08-MSM-60109807
n3:aktivita
n6:P n6:V n6:Z
n3:aktivity
P(QF4108), V, Z(MSM6010980701)
n3:dodaniDat
n7:2008
n3:domaciTvurceVysledku
n9:9759468 n9:9792759
n3:druhVysledku
n17:O
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
408375
n3:idVysledku
RIV/60109807:_____/07:#0000073
n3:jazykVysledku
n14:eng
n3:klicovaSlova
acclimatization; somatic hybrid; Solanum
n3:klicoveSlovo
n16:acclimatization n16:somatic%20hybrid n16:Solanum
n3:kontrolniKodProRIV
[E280681AA788]
n3:obor
n11:GE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n15:QF4108
n3:rokUplatneniVysledku
n7:2007
n3:tvurceVysledku
Polzerová, Hana Greplová, Marie
n3:zamer
n8:MSM6010980701