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Statements

Subject Item
n2:RIV%2F60077387%3A_____%2F03%3A62033016%21RIV%2F2004%2FAV0%2FA62004%2FN
rdf:type
skos:Concept n13:Vysledek
dcterms:description
ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Plešné Lake (pH about 5; high phosphatase activity) and eutrophic Římov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Plešné Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in Římov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1). ELF97 phosphate is a phosphatase substrate which produces ELFA, a fluorescent water-insoluble product, upon hydrolysis. We studied the kinetics of ELFA precipitation in acidic Plešné Lake (pH about 5; high phosphatase activity) and eutrophic Římov reservoir (pH 7-10; moderate phosphatase activity). Relative fluorescence ELFA precipitates was quantified with image analysis. Time-courses of ELFA formation exhibited lag periods followed by finite periods of linear increase. In Plešné Lake, lag-times were 1-18 min and rates of increase in ELFA fluorescence higher (by about 2 orders of magnitude) than in Římov reservoir (lag-times 30-200 min). Linear regression of seasonal data on rates of increase in ELFA fluorescence from image cytometry and spectrofluorometry (r (2) = 0.65, n = 10) allowed for calibration of image cytometry. Preliminary measurements of extracellular phosphatase activities of several algae resulted in rates 10-2260 fmol cell(-1) h(-1).
dcterms:title
Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics.
skos:prefLabel
Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics.
skos:notation
RIV/60077387:_____/03:62033016!RIV/2004/AV0/A62004/N
n3:strany
462;472
n3:aktivita
n10:Z n10:P
n3:aktivity
P(GA206/00/0063), P(IAA6017202), P(IBS6017004), Z(AV0Z6017912)
n3:cisloPeriodika
6
n3:dodaniDat
n11:2004
n3:domaciTvurceVysledku
n6:6252591 n6:4204859 n6:9408150 n6:9040358 n6:7264798
n3:druhVysledku
n17:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
606951
n3:idVysledku
RIV/60077387:_____/03:62033016
n3:jazykVysledku
n16:eng
n3:klicovaSlova
phosphorus; phytoplankton; lakes
n3:klicoveSlovo
n4:lakes n4:phytoplankton n4:phosphorus
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[B788155F8988]
n3:nazevZdroje
Environmental Microbiology
n3:obor
n15:EE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n8:GA206%2F00%2F0063 n8:IAA6017202 n8:IBS6017004
n3:rokUplatneniVysledku
n11:2003
n3:svazekPeriodika
5
n3:tvurceVysledku
Komárková, Jaroslava Štrojsová, Alena Nedoma, Jiří Šimek, Karel Vrba, Jaroslav
n3:zamer
n14:AV0Z6017912
s:issn
1462-2912
s:numberOfPages
11