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Statements

Subject Item
n2:RIV%2F60077387%3A_____%2F01%3A62013084%21RIV%2F2003%2FAV0%2FA62003%2FN
rdf:type
skos:Concept n13:Vysledek
dcterms:description
Direct inhibiting effects of Al on extracellular phosphatases produced by the plankton were studied in lab experiments. Significant reduction of an apparent substrate affinity was found at Al concentrations 300-1000 g l<sup>-1</sup> at pH 4.5 and 4.8, while maximum acid phosphatase activity (APA) remained unchanged. Such behaviour of saturation kinetics is compatible with the assumption that ionic Al acts as a competitive inhibitor. To decide whether the observed Al effects could be explained alternatively by complexation of Al with substrate, the best fits of data with both possible models was statistically tested. Experimental results supported the competitive hypothesis rather than the complexation model. For pH values <5.2, the results of an Al-pH matrix experiment gave a more detailed picture: the higher the Al concentration, the wider was the pH range where Al could negatively affect APA. The ecological ramifications of this effect were evaluated in the context of field APA data on three strong Direct inhibiting effects of Al on extracellular phosphatases produced by the plankton were studied in lab experiments. Significant reduction of an apparent substrate affinity was found at Al concentrations 300-1000 g l<sup>-1</sup> at pH 4.5 and 4.8, while maximum acid phosphatase activity (APA) remained unchanged. Such behaviour of saturation kinetics is compatible with the assumption that ionic Al acts as a competitive inhibitor. To decide whether the observed Al effects could be explained alternatively by complexation of Al with substrate, the best fits of data with both possible models was statistically tested. Experimental results supported the competitive hypothesis rather than the complexation model. For pH values <5.2, the results of an Al-pH matrix experiment gave a more detailed picture: the higher the Al concentration, the wider was the pH range where Al could negatively affect APA. The ecological ramifications of this effect were evaluated in the context of field APA data on three strong
dcterms:title
Impact of ionic aluminium on extracellular phosphatases in acidified lakes. Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
skos:prefLabel
Impact of ionic aluminium on extracellular phosphatases in acidified lakes. Impact of ionic aluminium on extracellular phosphatases in acidified lakes.
skos:notation
RIV/60077387:_____/01:62013084!RIV/2003/AV0/A62003/N
n4:strany
578;587
n4:aktivita
n14:P n14:Z
n4:aktivity
P(GA206/00/0063), P(GA206/97/0072), Z(AV0Z6017912)
n4:cisloPeriodika
9
n4:dodaniDat
n9:2003
n4:domaciTvurceVysledku
n5:7264798 n5:4286731 n5:9040358
n4:druhVysledku
n8:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
682452
n4:idVysledku
RIV/60077387:_____/01:62013084
n4:jazykVysledku
n6:eng
n4:klicovaSlova
acid phosphatases; pH effect; inhibition
n4:klicoveSlovo
n10:inhibition n10:pH%20effect n10:acid%20phosphatases
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[CF4DE69EEB10]
n4:nazevZdroje
Environmental Microbiology
n4:obor
n16:DA
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:pocetUcastnikuAkce
0
n4:pocetZahranicnichUcastnikuAkce
0
n4:projekt
n7:GA206%2F97%2F0072 n7:GA206%2F00%2F0063
n4:rokUplatneniVysledku
n9:2001
n4:svazekPeriodika
3
n4:tvurceVysledku
Kopáček, Jiří Bittl, T. Vrba, Jaroslav Nedoma, Jiří
n4:zamer
n11:AV0Z6017912
s:issn
1462-2912
s:numberOfPages
10