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Statements

Subject Item
n2:RIV%2F60077379%3A_____%2F02%3A61033052%21RIV%2F2004%2FAV0%2FA61004%2FN
rdf:type
skos:Concept n16:Vysledek
dcterms:description
We adapted the amplified ribosomal DNA restriction analysis (ARDRA) to examine the bacterial communities associated with the soil dwelling larvae of Penthetria holosericea (Diptera) and with vermicompost and earthworm (Eisenia andrei) gut content. Following DNA isolation and PCR amplification of the 16S rDNA, three restriction enzymes were used to generate ARDRA patterns. Resulting fragments were separated in a 1,5% agarose gel. When the universal eubacterial primers 63f and 1387r were used, the ARDRA patterns proved to be complex and difficult to resolve, although some minor differences were noted. The use of taxon specific PCR primers resulted in less complicated patterns and more differences in the band composition. The method is fast, cheap and useful in the characterization of bacterial communities. We adapted the amplified ribosomal DNA restriction analysis (ARDRA) to examine the bacterial communities associated with the soil dwelling larvae of Penthetria holosericea (Diptera) and with vermicompost and earthworm (Eisenia andrei) gut content. Following DNA isolation and PCR amplification of the 16S rDNA, three restriction enzymes were used to generate ARDRA patterns. Resulting fragments were separated in a 1,5% agarose gel. When the universal eubacterial primers 63f and 1387r were used, the ARDRA patterns proved to be complex and difficult to resolve, although some minor differences were noted. The use of taxon specific PCR primers resulted in less complicated patterns and more differences in the band composition. The method is fast, cheap and useful in the characterization of bacterial communities.
dcterms:title
Amplified Ribosomal DNA Restriction Analysis: experimental approach and procedures. Amplified Ribosomal DNA Restriction Analysis: experimental approach and procedures.
skos:prefLabel
Amplified Ribosomal DNA Restriction Analysis: experimental approach and procedures. Amplified Ribosomal DNA Restriction Analysis: experimental approach and procedures.
skos:notation
RIV/60077379:_____/02:61033052!RIV/2004/AV0/A61004/N
n4:strany
5;7
n4:aktivita
n15:Z n15:P
n4:aktivity
P(IAA6066001), P(IAB6066903), Z(AV0Z6066911)
n4:dodaniDat
n7:2004
n4:domaciTvurceVysledku
n19:7237243
n4:druhVysledku
n8:D
n4:duvernostUdaju
n18:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
637917
n4:idVysledku
RIV/60077379:_____/02:61033052
n4:jazykVysledku
n11:eng
n4:klicovaSlova
ARDRA; experimental approach
n4:klicoveSlovo
n6:ARDRA n6:experimental%20approach
n4:kontrolniKodProRIV
[73A03E924CB0]
n4:mistoKonaniAkce
Bratislava [SK]
n4:mistoVydani
Bratislava
n4:nazevZdroje
Život v pode. 3. medzinárodný seminár, sborník příspěvků na CD.
n4:obor
n10:EH
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
2
n4:pocetUcastnikuAkce
0
n4:pocetZahranicnichUcastnikuAkce
0
n4:projekt
n9:IAB6066903 n9:IAA6066001
n4:rokUplatneniVysledku
n7:2002
n4:tvurceVysledku
Oravecz, O. Krištůfek, Václav
n4:typAkce
n17:EUR
n4:zahajeniAkce
2002-01-29+01:00
n4:zamer
n20:AV0Z6066911
s:numberOfPages
3
n12:hasPublisher
Československá spoločnosť mikrobiologická