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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F14%3A00435256%21RIV15-GA0-60077344
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Tick-borne encephalitis (TBE), a disease caused by tick-borne encephalitis virus (TBEV), represents the most important flaviviral neural infection in Europe and north-eastern Asia. In the central nervous system (CNS), neurons are the primary target for TBEV infection; however, infection of non-neuronal CNS cells, such as astrocytes, is not well understood. In this study, we investigated the interaction between TBEV and primary human astrocytes. We report for the first time, to the best of our knowledge, that primary human astrocytes are sensitive to TBEV infection, although the infection did not affect their viability. The infection induced a marked increase in the expression of glial fibrillary acidic protein, a marker of astrocyte activation. In addition, expression of matrix metalloproteinase 9 and several key pro-inflammatory cytokines/chemokines (e.g. tumour necrosis factor alpha, interferon alpha, interleukin (IL)-1beta, IL-6, IL-8, interferon gamma-induced protein 10, macrophage inflammatory protein, but not monocyte chemotactic protein 1) was upregulated. Moreover, we present a detailed description of morphological changes in TBEV-infected cells, as investigated using three-dimensional electron tomography. Several novel ultrastructural changes were observed, including the formation of unique tubule-like structures of 17.9 0.15 nm diameter with associated viral particles and/or virus-induced vesicles and located in the rough endoplasmic reticulum of the TBEV-infected cells. This is the first demonstration that TBEV infection activates primary human astrocytes. The infected astrocytes might be a potential source of pro-inflammatory cytokines in the TBEV-infected brain, and might contribute to the TBEV-induced neurotoxicity and blood-brain barrier breakdown that occurs during TBE. The neuropathological significance of our observations is also discussed. Tick-borne encephalitis (TBE), a disease caused by tick-borne encephalitis virus (TBEV), represents the most important flaviviral neural infection in Europe and north-eastern Asia. In the central nervous system (CNS), neurons are the primary target for TBEV infection; however, infection of non-neuronal CNS cells, such as astrocytes, is not well understood. In this study, we investigated the interaction between TBEV and primary human astrocytes. We report for the first time, to the best of our knowledge, that primary human astrocytes are sensitive to TBEV infection, although the infection did not affect their viability. The infection induced a marked increase in the expression of glial fibrillary acidic protein, a marker of astrocyte activation. In addition, expression of matrix metalloproteinase 9 and several key pro-inflammatory cytokines/chemokines (e.g. tumour necrosis factor alpha, interferon alpha, interleukin (IL)-1beta, IL-6, IL-8, interferon gamma-induced protein 10, macrophage inflammatory protein, but not monocyte chemotactic protein 1) was upregulated. Moreover, we present a detailed description of morphological changes in TBEV-infected cells, as investigated using three-dimensional electron tomography. Several novel ultrastructural changes were observed, including the formation of unique tubule-like structures of 17.9 0.15 nm diameter with associated viral particles and/or virus-induced vesicles and located in the rough endoplasmic reticulum of the TBEV-infected cells. This is the first demonstration that TBEV infection activates primary human astrocytes. The infected astrocytes might be a potential source of pro-inflammatory cytokines in the TBEV-infected brain, and might contribute to the TBEV-induced neurotoxicity and blood-brain barrier breakdown that occurs during TBE. The neuropathological significance of our observations is also discussed.
dcterms:title
Infection and injury of human astrocytes by tick-borne encephalitis virus Infection and injury of human astrocytes by tick-borne encephalitis virus
skos:prefLabel
Infection and injury of human astrocytes by tick-borne encephalitis virus Infection and injury of human astrocytes by tick-borne encephalitis virus
skos:notation
RIV/60077344:_____/14:00435256!RIV15-GA0-60077344
n3:aktivita
n15:S n15:P n15:I
n3:aktivity
I, P(GAP302/12/2490), P(GAP502/11/2116), P(LO1218), P(TE01020118), S
n3:cisloPeriodika
Pt 11
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n9:7928114 n9:5089972 n9:8473900 n9:2749556 n9:9120998 n9:8966338
n3:druhVysledku
n16:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
21269
n3:idVysledku
RIV/60077344:_____/14:00435256
n3:jazykVysledku
n11:eng
n3:klicovaSlova
Tick-borne encephalitis; Tick-borne encephalitis virus; human
n3:klicoveSlovo
n4:human n4:Tick-borne%20encephalitis%20virus n4:Tick-borne%20encephalitis
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[C250E9D5294E]
n3:nazevZdroje
Journal of General Virology
n3:obor
n6:EE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
7
n3:projekt
n12:GAP302%2F12%2F2490 n12:LO1218 n12:GAP502%2F11%2F2116 n12:TE01020118
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
95
n3:tvurceVysledku
Elsterová, Jana Salát, J. Bílý, Tomáš Langhansová, Helena Vancová, Marie Palus, Martin Růžek, Daniel
n3:wos
000345309600009
s:issn
0022-1317
s:numberOfPages
16
n8:doi
10.1099/vir.0.068411-0