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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F14%3A00434798%21RIV15-GA0-60077344
rdf:type
skos:Concept n9:Vysledek
dcterms:description
Cytosolic and nuclear iron-sulphur (Fe/S) proteins include essential components involved in protein translation, DNA synthesis and DNA repair. In yeast and human cells, assembly of their Fe/S cofactor is accomplished by the CIA (cytosolic iron-sulphur protein assembly) machinery comprised of some 10 proteins. To investigate the extent of conservation of the CIA pathway, we examined its importance in the early-branching eukaryote Trypanosoma brucei that encodes all known CIA factors. Upon RNAi-mediated ablation of individual, early-acting CIA proteins, no major defects were observed in both procyclic and bloodstream stages. In contrast, parallel depletion of two CIA components was lethal, and severely diminished cytosolic aconitase activity lending support for a direct role of the CIA proteins in cytosolic Fe/S protein biogenesis. In support of this conclusion, the T. bruceiCIA proteins complemented the growth defects of their respective yeast CIA depletion mutants. Finally, the T. bruceiCIA factor Tah18 was characterized as a flavoprotein, while its binding partner Dre2 functions as a Fe/S protein. Together, our results demonstrate the essential and conserved function of the CIA pathway in cytosolic Fe/S protein assembly in both developmental stages of this representative of supergroup Excavata. Cytosolic and nuclear iron-sulphur (Fe/S) proteins include essential components involved in protein translation, DNA synthesis and DNA repair. In yeast and human cells, assembly of their Fe/S cofactor is accomplished by the CIA (cytosolic iron-sulphur protein assembly) machinery comprised of some 10 proteins. To investigate the extent of conservation of the CIA pathway, we examined its importance in the early-branching eukaryote Trypanosoma brucei that encodes all known CIA factors. Upon RNAi-mediated ablation of individual, early-acting CIA proteins, no major defects were observed in both procyclic and bloodstream stages. In contrast, parallel depletion of two CIA components was lethal, and severely diminished cytosolic aconitase activity lending support for a direct role of the CIA proteins in cytosolic Fe/S protein biogenesis. In support of this conclusion, the T. bruceiCIA proteins complemented the growth defects of their respective yeast CIA depletion mutants. Finally, the T. bruceiCIA factor Tah18 was characterized as a flavoprotein, while its binding partner Dre2 functions as a Fe/S protein. Together, our results demonstrate the essential and conserved function of the CIA pathway in cytosolic Fe/S protein assembly in both developmental stages of this representative of supergroup Excavata.
dcterms:title
Cytosolic iron-sulphur protein assembly is functionally conserved and essential in procyclic and bloodstream Trypanosoma brucei Cytosolic iron-sulphur protein assembly is functionally conserved and essential in procyclic and bloodstream Trypanosoma brucei
skos:prefLabel
Cytosolic iron-sulphur protein assembly is functionally conserved and essential in procyclic and bloodstream Trypanosoma brucei Cytosolic iron-sulphur protein assembly is functionally conserved and essential in procyclic and bloodstream Trypanosoma brucei
skos:notation
RIV/60077344:_____/14:00434798!RIV15-GA0-60077344
n4:aktivita
n5:S n5:P n5:I
n4:aktivity
I, P(EE2.3.30.0032), P(GAP305/11/2179), P(LH12104), S
n4:cisloPeriodika
5
n4:dodaniDat
n8:2015
n4:domaciTvurceVysledku
Basu, Somuvro n17:1369490
n4:druhVysledku
n7:J
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n14:predkladatel
n4:idSjednocenehoVysledku
9558
n4:idVysledku
RIV/60077344:_____/14:00434798
n4:jazykVysledku
n15:eng
n4:klicovaSlova
inducible expression system; Cfd1- Nbp35 complex; DNA metabolism; Fe/S proteins; transfer-RNA; cluster; mitochondrial; maturation; biogenesis; yeast
n4:klicoveSlovo
n6:maturation n6:transfer-RNA n6:Cfd1-%20Nbp35%20complex n6:DNA%20metabolism n6:cluster n6:Fe%2FS%20proteins n6:biogenesis n6:mitochondrial n6:yeast n6:inducible%20expression%20system
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[229804034055]
n4:nazevZdroje
Molecular Microbiology
n4:obor
n13:EB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
8
n4:projekt
n16:LH12104 n16:GAP305%2F11%2F2179 n16:EE2.3.30.0032
n4:rokUplatneniVysledku
n8:2014
n4:svazekPeriodika
93
n4:tvurceVysledku
Lill, R. Lukeš, Julius Basu, Somuvro Pierik, A. J. Netz, D. J. Lagny, T. J. Haindrich, A. C. Herlerth, N.
n4:wos
000341639600005
s:issn
0950-382X
s:numberOfPages
14
n18:doi
10.1111/mmi.12706