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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F14%3A00430609%21RIV15-GA0-60077344
rdf:type
skos:Concept n10:Vysledek
dcterms:description
The hard-bodied tick Ixodes ricinus (castor bean tick) is the most common tick species in Europe.I. ricinus is a vector of the causative agents of diseases that affect humans and animals including tick-borne encephalitis, borreliosis, tick-borne fever and babesiosis. The innate immune system provides ticks with quite an efficient defence against some pathogenic microorganisms in the event of their penetration into the tick body or through the blood meal. Antimicrobial peptides (AMPs) constitute an important feature of the tick immune system. Defensins are a well-known class of AMPs. Members of the defensin family of proteins have been reported in several tick species. So far, only two defensins had been identified from I. ricinus. In this study, we report the identification of six novel putative defensins from I. ricinus at the genomic and transcriptional levels. At the genomic level they show differences with one being intronless, while others contain two introns. The expression pattern of these molecules in the salivary glands, midgut, ovary, Malpighian tubules, haemolymph and the tick cell line IRE/CTVM19 was determined. Some of them are tissue specific while others seem to be ubiquitous. Molecular and phylogenetic analyses show that these novel members of the I. ricinus defensin family differ phylogenetically and structurally; nevertheless, the cysteine pattern is highly conserved among the family members. Finally, antimicrobial-peptide prediction tools were used to predict putative antimicrobial activity of our defensins. They show putative antimicrobial activity mainly against Gram-positive bacteria. This study displays the diversity of the defensin family in the tick I. ricinus. The hard-bodied tick Ixodes ricinus (castor bean tick) is the most common tick species in Europe.I. ricinus is a vector of the causative agents of diseases that affect humans and animals including tick-borne encephalitis, borreliosis, tick-borne fever and babesiosis. The innate immune system provides ticks with quite an efficient defence against some pathogenic microorganisms in the event of their penetration into the tick body or through the blood meal. Antimicrobial peptides (AMPs) constitute an important feature of the tick immune system. Defensins are a well-known class of AMPs. Members of the defensin family of proteins have been reported in several tick species. So far, only two defensins had been identified from I. ricinus. In this study, we report the identification of six novel putative defensins from I. ricinus at the genomic and transcriptional levels. At the genomic level they show differences with one being intronless, while others contain two introns. The expression pattern of these molecules in the salivary glands, midgut, ovary, Malpighian tubules, haemolymph and the tick cell line IRE/CTVM19 was determined. Some of them are tissue specific while others seem to be ubiquitous. Molecular and phylogenetic analyses show that these novel members of the I. ricinus defensin family differ phylogenetically and structurally; nevertheless, the cysteine pattern is highly conserved among the family members. Finally, antimicrobial-peptide prediction tools were used to predict putative antimicrobial activity of our defensins. They show putative antimicrobial activity mainly against Gram-positive bacteria. This study displays the diversity of the defensin family in the tick I. ricinus.
dcterms:title
Identification and partial characterisation of new members of the Ixodes ricinus defensin family Identification and partial characterisation of new members of the Ixodes ricinus defensin family
skos:prefLabel
Identification and partial characterisation of new members of the Ixodes ricinus defensin family Identification and partial characterisation of new members of the Ixodes ricinus defensin family
skos:notation
RIV/60077344:_____/14:00430609!RIV15-GA0-60077344
n3:aktivita
n18:P n18:I
n3:aktivity
I, P(EE2.3.30.0032), P(GAP302/11/1901)
n3:cisloPeriodika
2
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n4:5005116 Valdés, James J. n4:7790848 Tonk, Miray n4:2937204 Cabezas Cruz, Alejandro n4:9286357
n3:druhVysledku
n16:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
20257
n3:idVysledku
RIV/60077344:_____/14:00430609
n3:jazykVysledku
n5:eng
n3:klicovaSlova
antimicrobial peptide; defensin; Ixodes ricinus; tick; tick cell line
n3:klicoveSlovo
n6:Ixodes%20ricinus n6:antimicrobial%20peptide n6:tick%20cell%20line n6:tick n6:defensin
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[F0098DD05CC8]
n3:nazevZdroje
Gene
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
9
n3:projekt
n14:EE2.3.30.0032 n14:GAP302%2F11%2F1901
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
540
n3:tvurceVysledku
Golovchenko, Maryna de la Fuente, J. Tonk, Miray Cabezas Cruz, Alejandro Rego, Ryan O. M. Valdés, James J. Rudenko, Natalia Grubhoffer, Libor Bell-Sakyi, L.
n3:wos
000335102200003
s:issn
0378-1119
s:numberOfPages
7
n12:doi
10.1016/j.gene.2014.03.002