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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F14%3A00429865%21RIV15-GA0-60077344
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://onlinelibrary.wiley.com/doi/10.1002/jssc.201400113/pdf
dcterms:description
The quantification of phospholipid classes and the determination of their molecular structures are crucial in physiological and medical studies. This paper’s target analytes are cell membrane phospholipids, which play an important role in the seasonal acclimation processes of poikilothermic organisms.Weintroduce a set of simple and cost-effective analytical methods that enable efficient characterization and quantification of particular phospholipid classes and the identification and relative distribution of the individual phospholipid species. The analytical approach involves solid-phase extraction and high-performance thin-layer chromatography, which facilitate the separation of particular lipid classes. The obtained fractions are further transesterified to fatty acid methyl esters and subjected to gas chromatography coupled to flame ionization detection, which enables the determination of the position of double bonds. Phospholipid species separation is achieved by high-performance liquid chromatography with mass spectrometry, which gives information about the headgroup moiety and attached fatty acids. The total content of each phospholipids class is assessed by phosphorus determination by UV spectrophotometry. The simultaneous analysis of phosphorus, fatty acid residues, and phospholipid species provides detailed information about phospholipid composition. Evaluation of these coupled methods was achieved by application to an insect model, Pyrrhocoris apterus. High correlation was observed between fatty acid compositions as determined by gas chromatography and high-performance liquid chromatography analysis. The quantification of phospholipid classes and the determination of their molecular structures are crucial in physiological and medical studies. This paper’s target analytes are cell membrane phospholipids, which play an important role in the seasonal acclimation processes of poikilothermic organisms.Weintroduce a set of simple and cost-effective analytical methods that enable efficient characterization and quantification of particular phospholipid classes and the identification and relative distribution of the individual phospholipid species. The analytical approach involves solid-phase extraction and high-performance thin-layer chromatography, which facilitate the separation of particular lipid classes. The obtained fractions are further transesterified to fatty acid methyl esters and subjected to gas chromatography coupled to flame ionization detection, which enables the determination of the position of double bonds. Phospholipid species separation is achieved by high-performance liquid chromatography with mass spectrometry, which gives information about the headgroup moiety and attached fatty acids. The total content of each phospholipids class is assessed by phosphorus determination by UV spectrophotometry. The simultaneous analysis of phosphorus, fatty acid residues, and phospholipid species provides detailed information about phospholipid composition. Evaluation of these coupled methods was achieved by application to an insect model, Pyrrhocoris apterus. High correlation was observed between fatty acid compositions as determined by gas chromatography and high-performance liquid chromatography analysis.
dcterms:title
Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects
skos:prefLabel
Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: Application to insects
skos:notation
RIV/60077344:_____/14:00429865!RIV15-GA0-60077344
n3:aktivita
n5:P n5:I
n3:aktivity
I, P(GA13-18509S), P(GAP502/10/1612), P(TA01020969)
n3:cisloPeriodika
15
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n9:9968709 n9:2540258 n9:9354808 n9:8519064 n9:2946041
n3:druhVysledku
n17:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
9057
n3:idVysledku
RIV/60077344:_____/14:00429865
n3:jazykVysledku
n10:eng
n3:klicovaSlova
insects; mass spectrometry; phospholipids
n3:klicoveSlovo
n6:insects n6:mass%20spectrometry n6:phospholipids
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[3C7A1716B1A3]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:projekt
n4:TA01020969 n4:GAP502%2F10%2F1612 n4:GA13-18509S
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
37
n3:tvurceVysledku
Zahradníčková, Helena Berková, Petra Šimek, Petr Tomčala, Aleš Hodková, Magdalena Okrouhlík, J. Schneedorferová, Ivana
n3:wos
000339670600021
s:issn
1615-9306
s:numberOfPages
7
n19:doi
10.1002/jssc.201400113