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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F14%3A00429751%21RIV15-AV0-60077344
rdf:type
skos:Concept n15:Vysledek
dcterms:description
The saprophagous larvae of bibionid flies harbor bacteria in their alkaline intestinal tracts, but little is known about the contribution of the gut microbiota to the digestion of their recalcitrant diet. In this study, we measured oxygen and hydrogen partial pressure, redox potential and pH in the midgut, gastric caeca and hindgut of larvae of the bibionid fly Penthetria holosericea with Clark-type O2 and H2 microsensors, platinum redox microelectrodes, and LIX-type pH microelectrodes. The center of the midgut lumen was anoxic, whereas gastric caeca and hindgut were hypoxis. However, redox potential profiles indicated oxidizing conditions throughout the gut, with lowest values in the midgut (+20 to +60 mV). Hydrogen production was not detected. The midgut was extremely alkaline (pH around 11), whereas hindgut and gastric caeca were neutral to slightly alkaline. While HPLC analysis showed high concentrations of glucose in the midgut (15 mM) and gastric caeca (27 mM), the concentrations of microbial fermentation products such as lactate (2-4 mM), acetate (<1 mM) and succinate (<0,5 mM) were low in all gut regions, suggesting that the contribution of microorganisms to the digestive proces, particularly in the alkaline midgut, is only of minor importance. We conclude that the digestive strategy of the saprophytic larvae of P. holosericea, which feeds selectively on decomposed leaves and its own microbe-rich faeces, differs fundamentally from those of detritivorous and humivorous insects, which host a highly active, fermentative microbiota in their alkaline midgut or hindgut compartments. The saprophagous larvae of bibionid flies harbor bacteria in their alkaline intestinal tracts, but little is known about the contribution of the gut microbiota to the digestion of their recalcitrant diet. In this study, we measured oxygen and hydrogen partial pressure, redox potential and pH in the midgut, gastric caeca and hindgut of larvae of the bibionid fly Penthetria holosericea with Clark-type O2 and H2 microsensors, platinum redox microelectrodes, and LIX-type pH microelectrodes. The center of the midgut lumen was anoxic, whereas gastric caeca and hindgut were hypoxis. However, redox potential profiles indicated oxidizing conditions throughout the gut, with lowest values in the midgut (+20 to +60 mV). Hydrogen production was not detected. The midgut was extremely alkaline (pH around 11), whereas hindgut and gastric caeca were neutral to slightly alkaline. While HPLC analysis showed high concentrations of glucose in the midgut (15 mM) and gastric caeca (27 mM), the concentrations of microbial fermentation products such as lactate (2-4 mM), acetate (<1 mM) and succinate (<0,5 mM) were low in all gut regions, suggesting that the contribution of microorganisms to the digestive proces, particularly in the alkaline midgut, is only of minor importance. We conclude that the digestive strategy of the saprophytic larvae of P. holosericea, which feeds selectively on decomposed leaves and its own microbe-rich faeces, differs fundamentally from those of detritivorous and humivorous insects, which host a highly active, fermentative microbiota in their alkaline midgut or hindgut compartments.
dcterms:title
Microprofiles of oxygen, redox potential, and pH, and microbial fermentation products in the highly alkaline gut of the saprophagous larva of Penthetria holosericea (Diptera: Bibionidae) Microprofiles of oxygen, redox potential, and pH, and microbial fermentation products in the highly alkaline gut of the saprophagous larva of Penthetria holosericea (Diptera: Bibionidae)
skos:prefLabel
Microprofiles of oxygen, redox potential, and pH, and microbial fermentation products in the highly alkaline gut of the saprophagous larva of Penthetria holosericea (Diptera: Bibionidae) Microprofiles of oxygen, redox potential, and pH, and microbial fermentation products in the highly alkaline gut of the saprophagous larva of Penthetria holosericea (Diptera: Bibionidae)
skos:notation
RIV/60077344:_____/14:00429751!RIV15-AV0-60077344
n3:aktivita
n8:I
n3:aktivity
I
n3:cisloPeriodika
August
n3:dodaniDat
n9:2015
n3:domaciTvurceVysledku
n12:7039603
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
29200
n3:idVysledku
RIV/60077344:_____/14:00429751
n3:jazykVysledku
n13:eng
n3:klicovaSlova
dipteran larvae; gut pH; redox potential; oxygen partial pressure; hydrogen partial pressure
n3:klicoveSlovo
n6:dipteran%20larvae n6:oxygen%20partial%20pressure n6:hydrogen%20partial%20pressure n6:redox%20potential n6:gut%20pH
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[326E067A5531]
n3:nazevZdroje
Journal of Insect Physiology
n3:obor
n17:EG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n9:2014
n3:svazekPeriodika
67
n3:tvurceVysledku
Šustr, Vladimír Stingl, U. Brune, A.
n3:wos
000340326900008
s:issn
0022-1910
s:numberOfPages
6
n11:doi
10.1016/j.jinsphys.2014.06.007