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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F14%3A00427515%21RIV15-GA0-60077344
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Arthropod ecology and taxonomy have witnessed a substantial increase in the use of various trapping and molecular methods in the past two decades. However, the methods of collecting and the preservation liquids most widely used in arthropod traps do not properly preserve DNA. A stable and cheap substance that can be used to trap arthropods in and preserve their DNA is therefore needed. We test whether SDS/EDTA water solution can preserve DNA of beetles for one, four and eight weeks. Preservation of DNA was tested using PCR amplification of parts of the mitochondrial cytochrome c oxidase I (Cox1) and nuclear 28S rRN A genes. All the solutions tested preserved DNA for at least up to eight weeks and we recommend 2% SDS and 100mM EDTA as a cheap, stable and easily transportable alternative to ethanol for preserving specimens and their DNA collected in the field. This solution is also suitable for using as the collection and preservation liquid in arthropod traps. Arthropod ecology and taxonomy have witnessed a substantial increase in the use of various trapping and molecular methods in the past two decades. However, the methods of collecting and the preservation liquids most widely used in arthropod traps do not properly preserve DNA. A stable and cheap substance that can be used to trap arthropods in and preserve their DNA is therefore needed. We test whether SDS/EDTA water solution can preserve DNA of beetles for one, four and eight weeks. Preservation of DNA was tested using PCR amplification of parts of the mitochondrial cytochrome c oxidase I (Cox1) and nuclear 28S rRN A genes. All the solutions tested preserved DNA for at least up to eight weeks and we recommend 2% SDS and 100mM EDTA as a cheap, stable and easily transportable alternative to ethanol for preserving specimens and their DNA collected in the field. This solution is also suitable for using as the collection and preservation liquid in arthropod traps.
dcterms:title
A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods
skos:prefLabel
A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods A goodbye letter to alcohol: An alternative method for field preservation of arthropod specimens and DNA suitable for mass collecting methods
skos:notation
RIV/60077344:_____/14:00427515!RIV15-GA0-60077344
n3:aktivita
n4:I n4:P
n3:aktivity
I, P(GA204/09/1667), P(GA206/09/0115), P(GAP504/12/1952), P(TA02021501)
n3:cisloPeriodika
2
n3:dodaniDat
n13:2015
n3:domaciTvurceVysledku
n6:2942984 n6:7591055 n6:1709976 n6:9350470 n6:4876954 n6:1369490
n3:druhVysledku
n18:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
756
n3:idVysledku
RIV/60077344:_____/14:00427515
n3:jazykVysledku
n7:eng
n3:klicovaSlova
insects; beetles; DNA preservation
n3:klicoveSlovo
n5:DNA%20preservation n5:beetles n5:insects
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[042B41C41B2F]
n3:nazevZdroje
European Journal of Entomology
n3:obor
n12:EG
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n11:TA02021501 n11:GA206%2F09%2F0115 n11:GA204%2F09%2F1667 n11:GAP504%2F12%2F1952
n3:rokUplatneniVysledku
n13:2014
n3:svazekPeriodika
111
n3:tvurceVysledku
Lukeš, Julius Drag, Lukáš Čížek, Lukáš Pokluda, Pavel Stříbrná, Eva Novotný, Vojtěch
n3:wos
000343664100003
s:issn
1210-5759
s:numberOfPages
5
n14:doi
10.14411/eje.2014.024