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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F13%3A00420947%21RIV14-AV0-60077344
rdf:type
skos:Concept n4:Vysledek
dcterms:description
Saliva is a key element of interaction between hematophagous mosquitoes and their vertebrate hosts. In addition to allowing a successful blood meal by neutralizing or delaying hemostatic responses, the salivary cocktail is also able to modulate the effector mechanisms of host immune responses facilitating, in turn, the transmission of several types of microorganisms. Understanding how the mosquito uses its salivary components to circumvent host immunity might help to clarify the mechanisms of transmission of such pathogens and disease establishment. Flow cytometry was used to evaluate if increasing concentrations of A. aegypti salivary gland extract (SGE) affects bone marrow-derived DC differentiation and maturation. Lymphocyte proliferation in the presence of SGE was estimated by a colorimetric assay. Western blot and Annexin V staining assays were used to assess apoptosis in these cells. Naive and memory cells from mosquito-bite exposed mice or OVA-immunized mice and their respective controls were analyzed by flow cytometry. Concentration-response curves were employed to evaluate A. aegypti SGE effects on DC and lymphocyte biology. DCs differentiation from bone marrow precursors, their maturation and function were not directly affected by A. aegypti SGE (concentrations ranging from 2.5 to 40 mug/mL). On the other hand, lymphocytes were very sensitive to the salivary components and died in the presence of A. aegypti SGE, even at concentrations as low as 0.1 mug/mL. In addition, A. aegypti SGE was shown to induce apoptosis in all lymphocyte populations evaluated (CD4+ and CD8+ T cells, and B cells) through a mechanism involving caspase-3 and caspase-8, but not Bim. By using different approaches to generate memory cells, we were able to verify that these cells are resistant to SGE effects. Our results show that lymphocytes, and not DCs, are the primary target of A. aegypti salivary components. Saliva is a key element of interaction between hematophagous mosquitoes and their vertebrate hosts. In addition to allowing a successful blood meal by neutralizing or delaying hemostatic responses, the salivary cocktail is also able to modulate the effector mechanisms of host immune responses facilitating, in turn, the transmission of several types of microorganisms. Understanding how the mosquito uses its salivary components to circumvent host immunity might help to clarify the mechanisms of transmission of such pathogens and disease establishment. Flow cytometry was used to evaluate if increasing concentrations of A. aegypti salivary gland extract (SGE) affects bone marrow-derived DC differentiation and maturation. Lymphocyte proliferation in the presence of SGE was estimated by a colorimetric assay. Western blot and Annexin V staining assays were used to assess apoptosis in these cells. Naive and memory cells from mosquito-bite exposed mice or OVA-immunized mice and their respective controls were analyzed by flow cytometry. Concentration-response curves were employed to evaluate A. aegypti SGE effects on DC and lymphocyte biology. DCs differentiation from bone marrow precursors, their maturation and function were not directly affected by A. aegypti SGE (concentrations ranging from 2.5 to 40 mug/mL). On the other hand, lymphocytes were very sensitive to the salivary components and died in the presence of A. aegypti SGE, even at concentrations as low as 0.1 mug/mL. In addition, A. aegypti SGE was shown to induce apoptosis in all lymphocyte populations evaluated (CD4+ and CD8+ T cells, and B cells) through a mechanism involving caspase-3 and caspase-8, but not Bim. By using different approaches to generate memory cells, we were able to verify that these cells are resistant to SGE effects. Our results show that lymphocytes, and not DCs, are the primary target of A. aegypti salivary components.
dcterms:title
Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology
skos:prefLabel
Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology
skos:notation
RIV/60077344:_____/13:00420947!RIV14-AV0-60077344
n4:predkladatel
n5:ico%3A60077344
n3:aktivita
n13:I
n3:aktivity
I
n3:cisloPeriodika
NOV 2013
n3:dodaniDat
n16:2014
n3:domaciTvurceVysledku
Kotsyfakis, Michalis
n3:druhVysledku
n12:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
71908
n3:idVysledku
RIV/60077344:_____/13:00420947
n3:jazykVysledku
n17:eng
n3:klicovaSlova
dendritic cells; T-cells; Aedes aegypti; saliva; apoptosis
n3:klicoveSlovo
n10:Aedes%20aegypti n10:saliva n10:dendritic%20cells n10:apoptosis n10:T-cells
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[4796B854577D]
n3:nazevZdroje
Parasites Vectors
n3:obor
n14:EC
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
11
n3:rokUplatneniVysledku
n16:2013
n3:svazekPeriodika
6
n3:tvurceVysledku
Maciel, C. Capurro, M. L. Barros, M. S. Campopiano, J. Gueroni, D.I. Sa-Nunes, A. Bizzarro, B. Lino, C.N. Kotsyfakis, Michalis Amarante-Mendes, G. P. Calvo, E.
n3:wos
000328833800001
s:issn
1756-3305
s:numberOfPages
13
n9:doi
10.1186/1756-3305-6-329