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Statements

Subject Item
n2:RIV%2F60077344%3A_____%2F13%3A00398087%21RIV14-AV0-60077344
rdf:type
skos:Concept n9:Vysledek
dcterms:description
Fertilizing soils with animal excrements from farms with common antibiotic use represents a risk of disseminating antibiotic resistance genes into the environment. In the case of tetracycline antibiotics, it is not clear, however, whether the presence of antibiotic residues further enhances the gene occurrence in manured soils. We established a microcosm experiment in which 3 farm soils that had no recent history of fertilization with animal excrements were amended on a weekly basis (9 times) with excrements from either an oxytetracycline-treated or an untreated cow. Throughout the study, the concentration of oxytetracycline in excrements from the treated cow was above 500 μg g-1 dw, whereas no oxytetracycline was detected in excrements from the healthy cow. Both excrements contained tetracycline resistance (TC-r) genes tet(L), tet(M), tet(V), tet(Z), tet(Q) and tet(W). The excrements from the treated cow also contained the tet(B) gene, and a higher abundance of tet(Z), tet(Q) and tet(W). Three weeks after the last excrement addition, the individual TC-r genes differed in their persistence in soil: tet(Q) and tet(B) were not detectable while tet(L), tet(M), tet(Z) and tet(W) were found in all 3 soils. There were, however, no significant differences in the total number, nor in the abundance, of TC-r genes between soil samples amended with each excrement type. The oxytetracycline-rich and the oxytetracycline-free excrement therefore contributed equally to the increase of tetracycline resistome in soil. Our results indicate that other mechanisms than OTC-selection pressure may be involved in the maintenance of TC-r genes in manured soils. Fertilizing soils with animal excrements from farms with common antibiotic use represents a risk of disseminating antibiotic resistance genes into the environment. In the case of tetracycline antibiotics, it is not clear, however, whether the presence of antibiotic residues further enhances the gene occurrence in manured soils. We established a microcosm experiment in which 3 farm soils that had no recent history of fertilization with animal excrements were amended on a weekly basis (9 times) with excrements from either an oxytetracycline-treated or an untreated cow. Throughout the study, the concentration of oxytetracycline in excrements from the treated cow was above 500 μg g-1 dw, whereas no oxytetracycline was detected in excrements from the healthy cow. Both excrements contained tetracycline resistance (TC-r) genes tet(L), tet(M), tet(V), tet(Z), tet(Q) and tet(W). The excrements from the treated cow also contained the tet(B) gene, and a higher abundance of tet(Z), tet(Q) and tet(W). Three weeks after the last excrement addition, the individual TC-r genes differed in their persistence in soil: tet(Q) and tet(B) were not detectable while tet(L), tet(M), tet(Z) and tet(W) were found in all 3 soils. There were, however, no significant differences in the total number, nor in the abundance, of TC-r genes between soil samples amended with each excrement type. The oxytetracycline-rich and the oxytetracycline-free excrement therefore contributed equally to the increase of tetracycline resistome in soil. Our results indicate that other mechanisms than OTC-selection pressure may be involved in the maintenance of TC-r genes in manured soils.
dcterms:title
Cow excrements enhance the occurrence of tetracycline resistance genes in soil regardless of their oxytetracycline content Cow excrements enhance the occurrence of tetracycline resistance genes in soil regardless of their oxytetracycline content
skos:prefLabel
Cow excrements enhance the occurrence of tetracycline resistance genes in soil regardless of their oxytetracycline content Cow excrements enhance the occurrence of tetracycline resistance genes in soil regardless of their oxytetracycline content
skos:notation
RIV/60077344:_____/13:00398087!RIV14-AV0-60077344
n9:predkladatel
n10:ico%3A60077344
n3:aktivita
n16:I n16:P
n3:aktivity
I, P(ED1.1.00/02.0073), P(EE2.3.30.0032), P(GAP504/10/2077)
n3:cisloPeriodika
10
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
n11:8144095 n11:9706100 n11:1850261 n11:4209125
n3:druhVysledku
n13:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
67228
n3:idVysledku
RIV/60077344:_____/13:00398087
n3:jazykVysledku
n14:eng
n3:klicovaSlova
cattle excrement; environmental risk assessment; manured soil; oxytetracycline; tetracycline resistance; tetracycline resistance gene
n3:klicoveSlovo
n7:tetracycline%20resistance n7:manured%20soil n7:tetracycline%20resistance%20gene n7:oxytetracycline n7:cattle%20excrement n7:environmental%20risk%20assessment
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[26818658CF60]
n3:nazevZdroje
Chemosphere
n3:obor
n8:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n5:ED1.1.00%2F02.0073 n5:GAP504%2F10%2F2077 n5:EE2.3.30.0032
n3:rokUplatneniVysledku
n4:2013
n3:svazekPeriodika
93
n3:tvurceVysledku
Bradley, R. Elhottová, Dana Chroňáková, Alica Schmitt, H. Kyselková, Martina Jirout, Jiří Vrchotová, Naděžda
n3:wos
000327566600027
s:issn
0045-6535
s:numberOfPages
6
n18:doi
10.1016/j.chemosphere.2013.08.058